Recent progress in quantitative proteomics using stable isotope labeling, multidimensional liquid chromatography and mass spectrometry

Jue Liang Hsu, Shu Hui Chen

研究成果: Review article同行評審

4 引文 斯高帕斯(Scopus)

摘要

Stable isotope labeling coupled with multidimensional liquid chromatography/tandem mass spectrometry is an advanced platform for global proteome-wide quantification. Compared to conventional methods, such as two dimensional polyacrylamide gel or electroblot based quantification methods, stable isotope labeling holds greater promise for accurate and large-scale quantitative analyses. Various labeling strategies have been developed to analyze protein expression, post-translational modification, and protein/protein interactions, as well as for absolute quantification. There are advantages and disadvantages inherent with each method, but their applicability is dependent on many factors. In addition to the choice of which labeling chemistry to be used, there are several bottleneck issues associated with this approach that are of critical importance. These include finding effective multidimensional separation steps to resolve low abundant proteins present in relatively complicated mixtures, validating the methods, and interpreting the large amount of statistical data generated. This review covers the current progress in solving these concerns and summarizes the various labeling strategies and applications. We believe that a judicious integration of each component of the technique is crucial for the success of such a global systems approach. Based on the current progress, it is clear that the stable isotope labeling/mass spectrometry technique will find tremendous use in many fields, such as drug discovery, clinical diagnostics, disease prevention, basic biological research, and biotechnology.

原文English
頁(從 - 到)287-302
頁數16
期刊Current Proteomics
2
發行號4
DOIs
出版狀態Published - 2005 12月

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學

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