Reduced expression of von Hippel-Lindau gene in subjects exposed to polychlorinated biphenyls and dibenzofurans

Pei Chien Tsai, Ying-Jan Wang, Jui He Tsai, Yueliang Leon Guo, Tzuu Huei Ueng, Hsiao-Sheng Liu, Wen-Ya Huang

研究成果: Article

7 引文 (Scopus)

摘要

Polychlorinated biphenyls (PCBs) and polychlorinated dibenzofurans (PCDFs) are ubiquitous pollutants found in the environment and human tissues. A cohort in Taiwan has undergone follow-up for 24 years after exposure to high levels of PCBs and PCDFs. The incidence of chloracne, hyperkeratosis, and abnormal nail was increased among exposed people. We conducted a study to identify the genes whose expressions were affected by such exposure. A cDNA microarray system consisting of 908 genes was used for pooled serum samples from non-smoking men exposed to PCBs and PCDFs (n=15) and their matched referents (n=15) in triplicate. After adjusting for background and housekeeping genes, genes with different expressions between the exposure and reference groups were determined by both regression and cluster analysis, and further confirmed by real-time RT-PCR. The tumor suppressor gene von Hippel-Lindau (VHL) was found to be down-regulated in the microarray analysis. VHL gene expression levels were also found to be positively associated with age, shown by real-time RT-PCR. Upon age adjustment, VHL gene expression was reduced in Yucheng ("oil disease") subjects as compared to referents. Among Yucheng people, those with abnormal nails had lower VHL expressions than those without abnormal nails. These findings provide new insights into the potential role of VHL in health conditions associated with PCB and PCDF exposures.

原文English
頁(從 - 到)247-251
頁數5
期刊Environmental Research
108
發行號2
DOIs
出版狀態Published - 2008 十月 1

指紋

Malformed Nails
Polychlorinated Biphenyls
PCDF
Nails
PCB
Genes
Gene expression
gene expression
gene
Microarrays
Gene Expression
Real-Time Polymerase Chain Reaction
Chloracne
Essential Genes
Cluster analysis
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Tumor Suppressor Genes
Taiwan
Regression analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Environmental Science(all)

引用此文

Tsai, Pei Chien ; Wang, Ying-Jan ; Tsai, Jui He ; Guo, Yueliang Leon ; Ueng, Tzuu Huei ; Liu, Hsiao-Sheng ; Huang, Wen-Ya. / Reduced expression of von Hippel-Lindau gene in subjects exposed to polychlorinated biphenyls and dibenzofurans. 於: Environmental Research. 2008 ; 卷 108, 編號 2. 頁 247-251.
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abstract = "Polychlorinated biphenyls (PCBs) and polychlorinated dibenzofurans (PCDFs) are ubiquitous pollutants found in the environment and human tissues. A cohort in Taiwan has undergone follow-up for 24 years after exposure to high levels of PCBs and PCDFs. The incidence of chloracne, hyperkeratosis, and abnormal nail was increased among exposed people. We conducted a study to identify the genes whose expressions were affected by such exposure. A cDNA microarray system consisting of 908 genes was used for pooled serum samples from non-smoking men exposed to PCBs and PCDFs (n=15) and their matched referents (n=15) in triplicate. After adjusting for background and housekeeping genes, genes with different expressions between the exposure and reference groups were determined by both regression and cluster analysis, and further confirmed by real-time RT-PCR. The tumor suppressor gene von Hippel-Lindau (VHL) was found to be down-regulated in the microarray analysis. VHL gene expression levels were also found to be positively associated with age, shown by real-time RT-PCR. Upon age adjustment, VHL gene expression was reduced in Yucheng ({"}oil disease{"}) subjects as compared to referents. Among Yucheng people, those with abnormal nails had lower VHL expressions than those without abnormal nails. These findings provide new insights into the potential role of VHL in health conditions associated with PCB and PCDF exposures.",
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Reduced expression of von Hippel-Lindau gene in subjects exposed to polychlorinated biphenyls and dibenzofurans. / Tsai, Pei Chien; Wang, Ying-Jan; Tsai, Jui He; Guo, Yueliang Leon; Ueng, Tzuu Huei; Liu, Hsiao-Sheng; Huang, Wen-Ya.

於: Environmental Research, 卷 108, 編號 2, 01.10.2008, p. 247-251.

研究成果: Article

TY - JOUR

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AU - Tsai, Pei Chien

AU - Wang, Ying-Jan

AU - Tsai, Jui He

AU - Guo, Yueliang Leon

AU - Ueng, Tzuu Huei

AU - Liu, Hsiao-Sheng

AU - Huang, Wen-Ya

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AB - Polychlorinated biphenyls (PCBs) and polychlorinated dibenzofurans (PCDFs) are ubiquitous pollutants found in the environment and human tissues. A cohort in Taiwan has undergone follow-up for 24 years after exposure to high levels of PCBs and PCDFs. The incidence of chloracne, hyperkeratosis, and abnormal nail was increased among exposed people. We conducted a study to identify the genes whose expressions were affected by such exposure. A cDNA microarray system consisting of 908 genes was used for pooled serum samples from non-smoking men exposed to PCBs and PCDFs (n=15) and their matched referents (n=15) in triplicate. After adjusting for background and housekeeping genes, genes with different expressions between the exposure and reference groups were determined by both regression and cluster analysis, and further confirmed by real-time RT-PCR. The tumor suppressor gene von Hippel-Lindau (VHL) was found to be down-regulated in the microarray analysis. VHL gene expression levels were also found to be positively associated with age, shown by real-time RT-PCR. Upon age adjustment, VHL gene expression was reduced in Yucheng ("oil disease") subjects as compared to referents. Among Yucheng people, those with abnormal nails had lower VHL expressions than those without abnormal nails. These findings provide new insights into the potential role of VHL in health conditions associated with PCB and PCDF exposures.

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