Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases

Muriel Bligny, Florence Courtois, Safia Thaminy, Ching Chun Chang, Thierry Lagrange, Jahnabi Baruah-Wolff, David Stern, Silva Lerbs-Mache

研究成果: Article同行評審

63 引文 斯高帕斯(Scopus)

摘要

The plastid genome is known to be transcribed by a plastid-encoded prokaryotic-type RNA polymerase (PEP) and by a nucleus-encoded phage-type RNA polymerase (NEP). The spinach plastid rrn operon promoter region harbours three different, overlapping promoters. Two of them are of the prokaryotic type. The third promoter is a non-consensus-type NEP promoter. We separated three different transcriptional activities from spinach chloroplasts: PEP, the phage-type RNA polymerase NEP-1, and a third, hitherto undescribed transcriptional activity (NEP-2). NEP-2 specifically transcribes the rrn operon in the presence of the transcription factor CDF2. CDF2 was previously shown to recruit PEP to the rrn promoter to repress transcription. Together, our results suggest the existence of a third RNA polymerase in plastids and a mechanism of rDNA transcriptional regulation that is based on the interaction of the transcription factor CDF2 with two different transcriptional systems.

原文English
頁(從 - 到)1851-1860
頁數10
期刊EMBO Journal
19
發行號8
DOIs
出版狀態Published - 2000 4月 17

All Science Journal Classification (ASJC) codes

  • 一般神經科學
  • 分子生物學
  • 一般生物化學,遺傳學和分子生物學
  • 一般免疫學和微生物學

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