TY - JOUR
T1 - Relationship between fibroblast growth factor 23 and biochemical and bone histomorphometric alterations in a chronic kidney disease rat model undergoing parathyroidectomy
AU - Liao, Hung Wei
AU - Hung, Peir Haur
AU - Hsiao, Chih Yen
AU - Liou, Hung Hsiang
AU - Lin, Hsin Shih
AU - Huang, Tsang Hai
AU - Jou, I. Ming
AU - Tsai, Kuen Jer
N1 - Funding Information:
This article is revised by M.D. Yu-Ru Liao, Physician of An-Li Pediatric and Medical Clinic, Pu-Li, Nantou, Taiwan. This study was partly supported by National Cheng Kung University Hospital (NCKUH-10408012) and National Cheng Kung University.
Publisher Copyright:
© 2015 Liao et al.
PY - 2015/7/17
Y1 - 2015/7/17
N2 - Background: Phosphate burden in chronic kidney disease (CKD) leads to elevated serum fibroblast factor- 23 (FGF-23) levels, secondary hyperparathyroidism and chronic kidney disease-mineral bone disorder (CKD-MBD). However dissociated hyperphosphatemia and low serum FGF- 23 concentrations have been observed in experimentally parathyoridectomized rats. The relationships between serum mineral, hormone, and bone metabolism may be altered in the presence of CKD. The aim of our study was to investigate whether a consistent relationship existed between serum FGF-23 levels, specific serum biochemical markers, and histomorphometric parameters of bone metabolism in a parathyroidectomized CKD animal model. Results: Sprague Dawley rats were divided into 3 groups: parathyroidectomy (PTX) and CKD (PTX +CKD, 9 rats), CKD without PTX (CKD, 9 rats), and neither PTX nor CKD (sham-operated control, 8 rats); CKD was induced by partial nephrectomy. At 8 weeks after partial nephrectomy, serum biomarkers were measured. Bone histomorphometries of the distal femoral metaphyseal bone were analyzed. The mean serum FGF-23 levels and mean bone formation rate were the highest in the CKD group and the lowest in the PTX+CKD group. Bone volume parameters increased significantly in the PTX+CKD group. Pearson's correlation revealed that serum FGF-23 levels associated with those of intact parathyroid hormone, phosphate, collagen type I C-telopeptide, and calcium. Univariate linear regression showed that serum FGF-23 values correlated with bone formation rate, bone volume, and osteoid parameters. Stepwise multivariate regression analysis revealed that circulating FGF-23 values were independently associated with bone volume and thickness (β = -0.737; p < 0.001 and β = -0.526; p = 0.006, respectively). Serum parathyroid hormone levels independently correlated with bone formation rate (β = 0.714; p < 0.001) while collagen type I C-telopeptide levels correlated with osteoid parameter. Conclusion: Serum FGF-23 levels independently correlated with bone volume parameters in rats with experimentally induced CKD.
AB - Background: Phosphate burden in chronic kidney disease (CKD) leads to elevated serum fibroblast factor- 23 (FGF-23) levels, secondary hyperparathyroidism and chronic kidney disease-mineral bone disorder (CKD-MBD). However dissociated hyperphosphatemia and low serum FGF- 23 concentrations have been observed in experimentally parathyoridectomized rats. The relationships between serum mineral, hormone, and bone metabolism may be altered in the presence of CKD. The aim of our study was to investigate whether a consistent relationship existed between serum FGF-23 levels, specific serum biochemical markers, and histomorphometric parameters of bone metabolism in a parathyroidectomized CKD animal model. Results: Sprague Dawley rats were divided into 3 groups: parathyroidectomy (PTX) and CKD (PTX +CKD, 9 rats), CKD without PTX (CKD, 9 rats), and neither PTX nor CKD (sham-operated control, 8 rats); CKD was induced by partial nephrectomy. At 8 weeks after partial nephrectomy, serum biomarkers were measured. Bone histomorphometries of the distal femoral metaphyseal bone were analyzed. The mean serum FGF-23 levels and mean bone formation rate were the highest in the CKD group and the lowest in the PTX+CKD group. Bone volume parameters increased significantly in the PTX+CKD group. Pearson's correlation revealed that serum FGF-23 levels associated with those of intact parathyroid hormone, phosphate, collagen type I C-telopeptide, and calcium. Univariate linear regression showed that serum FGF-23 values correlated with bone formation rate, bone volume, and osteoid parameters. Stepwise multivariate regression analysis revealed that circulating FGF-23 values were independently associated with bone volume and thickness (β = -0.737; p < 0.001 and β = -0.526; p = 0.006, respectively). Serum parathyroid hormone levels independently correlated with bone formation rate (β = 0.714; p < 0.001) while collagen type I C-telopeptide levels correlated with osteoid parameter. Conclusion: Serum FGF-23 levels independently correlated with bone volume parameters in rats with experimentally induced CKD.
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U2 - 10.1371/journal.pone.0133278
DO - 10.1371/journal.pone.0133278
M3 - Article
C2 - 26186634
AN - SCOPUS:84941312760
VL - 10
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 7
M1 - e0133278
ER -