Relationship Between Rectal Swab and Tissue Samples in Mucosa-associated Microbiota in Patients With Inflammatory Bowel Disease

Tsunglin Liu, Cheng Han Lin, Yi Lin Chen, Chien Cheng Yeh, Yuan Yow Chiou, Jiu-Yao Wang, Chung Ta Lee, Kuo Ting Lee, Ai-Li Shiau, Chao-Liang Wu, Chien Chin Chen, Jui Wen Kang, Po Jun Chen, Hsin Yu Kuo, Er Hsiang Yang, Chiao Hsiung Chuang

研究成果: Article同行評審

摘要

Background: Gut mucosa-associated microbiota is more closely correlated with disease phenotypes than fecal microbiota; however sampling via tissue biopsy is more invasive and uncomfortable. Rectal swab may be a suitable substitute for tissue biopsy, but its effectiveness is controversial. This study aimed to evaluate differences in the microbiota at these sites in patients with inflammatory bowel disease (IBD). Methods: Inflammatory bowel disease patients and a control group were enrolled when surveillance colonoscopy was scheduled. Samples of colon biopsy tissues, rectal swabs during colonoscopy, and feces before bowel preparation were collected to analyze microbial composition. To explore the short-term effects of bowel preparation on swab microbiota, prepreparation swab samples were also collected from 27 IBD patients. Results: A total of 33 Crohn’s disease, 54 ulcerative colitis, and 21 non-IBD patients were enrolled. In beta diversity analysis, fecal microbiota clearly differed from swab and tissue microbiota in the 3 disease groups. The swab microbiota was closer to, but still different from, the tissue microbiota. Consistently, we identified that swab samples differed more in abundant genera from feces than from tissue. Beta diversity analysis did not reveal a difference in swab microbiota before and after bowel preparation, but the genus composition of most individuals varied markedly. Conclusions: Swab microbiota more closely resembled tissue microbiota relative to fecal microbiota, but there were still differences. Bowel preparation did not alter the overall swab microbiota in the short term but markedly changed the microbial composition in most patients.

原文English
頁(從 - 到)447-458
頁數12
期刊Inflammatory Bowel Diseases
30
發行號3
DOIs
出版狀態Published - 2024 3月 1

All Science Journal Classification (ASJC) codes

  • 免疫學和過敏
  • 消化內科

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