Respiration-dependent membrane hyperpolarization in tonoplast-free cells of nitella axilliformis

Tetsuro Mimura, Teruo Shimmen, Masashi Tazawa

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)


Cells of Nitella axilliformis were made tonoplast-free by intracellular perfusion of media containing ethyleneglycol-bis-(β-aminoethylether)N,N'-tetraacetic acid (EGTA). When the perfusion medium contained ADP as well as ATP, the membrane hyperpolarized in darkness in a manner similar to light-induced hyperpolarization. This light-independent hyperpolarization seems to be due to activation of the electrogenic ion pump in the plasma membrane because the hyperpolarized value of the membrane potential was more negative than the equilibrium potential for K$, the most negative ion equilibrium potential in Nitella.The hyperpolarization was inhibited by the respiratory chain inhibitors NaCN (1 mM), antimycin A (10 μM) and rotenone (10 μM). NaCN slightly decreased the ATP concentration in the cell perfused with medium containing 1 mM ATP and 1 mM ADP; but, even after treatment with NaCN, the cell had about 80% of the ATP value for the control.

頁(從 - 到)1419-1425
期刊Plant and Cell Physiology
出版狀態Published - 1982 12月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 植物科學
  • 細胞生物學


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