RFLP and inheritance patterns of chloroplast DNA in intergeneric hybrids of Phalaenopsis and Doritis

Song-Bin Chang, Wen Huei Chen, Hong-Hwa Chen, Yang Ming Fu, Yih Shyan Lin

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24 引文 斯高帕斯(Scopus)

摘要

The mode of inheritance of chloroplasts was analyzed using restriction fragment length polymorphism (RFLP) in both interspecific hybrids of Phalaenopsis and intergeneric hybrids of Phalaenopsis and Doritis. Chloroplast DNA digested with Dra I followed by hybridization with an rbcL probe revealed that Phalaenopsis amabilis, Phalaenopsis aphrodite, and Phalaenopsis stuartiana, which belong to the taxonomic section PHALAENOPSIS, have the same size 2.0-kb fragment. Both Phalaenopsis mannii and Phalaenopsis amboinensis have a 2.3-kb fragment, while Doritis pulcherrima has a 3.5-kb fragment. In both interspecific and intergeneric hybrids, maternal inheritance of the chloroplast genome was detected. The hybrids of both reciprocal crosses (A x B and B x A) are registered with the same hybrid names in Sander's List of Orchid Hybrids at the Royal Horticultural Society, despite harboring chloroplast DNA from different parents. These results suggest that the chloroplast DNA can be used as a marker for identification of parentship and for phylogenetic studies of taxonomy.

原文English
頁(從 - 到)219-223
頁數5
期刊Botanical Bulletin of Academia Sinica
41
發行號3
出版狀態Published - 2000 七月

All Science Journal Classification (ASJC) codes

  • 植物科學

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