RGNNV induces mitochondria-mediated cell death via newly synthesized protein dependent pathway in fish cells

Horng Cherng Wu, Jen Leih Wu, Heuy Ling Chu, Yu Chin Su, Jiann Ruey Hong

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

The RNA nervous necrosis virus induces necrotic cell death in fish; however, the molecular mechanism remains unknown. In this study, we demonstrated that beta-nodavirus-induced mitochondria-mediated dependent cell death is through newly synthesized protein dependent pathway in replication cycle. We determined that newly synthesized protein dependent pathway is required for red-spotted grouper nervous necrosis virus (RGNNV)-induced cell death. UV irradiation of the virus effectively blocked viral replication and cell death. Next, RGNNV RNA-dependent RNA polymerase (RdRp or protein A) was cloned and its involvement in RNA genome replication and viral protein synthesis was analyzed. Protein A was initially expressed 48 h post-infection and localized to the cytoplasm. Knockdown of protein A expression completely blocked viral genomic replication and expression of viral protein expression RNA1 small hairpin RNA (shRNA) producing cell lines, which coincided with inhibition of phosphatidylserine exposure, mitochondria-mediated death signaling, and increased cell viability 72 h post-infection. Furthermore, RGNNV-induced mitochondria-mediated caspase-3-independent necrotic cell death is dependent on viral synthesized protein dependent pathway at the middle-late replication stage. Taken together, for instance these results suggested that RGNNV induces cell death may require newly synthesized protein for triggering host mitochondria-mediated cell death. These findings may provide new insights into RNA viral pathogenesis.

原文English
頁(從 - 到)451-463
頁數13
期刊Fish and Shellfish Immunology
29
發行號3
DOIs
出版狀態Published - 2010 九月

All Science Journal Classification (ASJC) codes

  • 環境化學
  • 海洋科學

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