Ripple structure-generated hybrid electrokinetics for on-chip mixing and separating of functionalized beads

I. Fang Cheng, Sheng Chuan Chiang, Cheng Che Chung, Trai-Ming Yeh, Hsien-Chang Chang

研究成果: Article

3 引文 (Scopus)

摘要

We present an electrokinetics-based microfluidic platform that is capable of on-chip manipulating, mixing, and separating microparticles through adjusting the interrelated magnitudes of dielectrophoresis and AC electroosmosis. Hybrid electrokinetic phenomenon is generated from an electric field-induced micro-ripple structure made of ultraviolet-curable glue. Size-dependent particle separation and selective removal over the ripple structure is demonstrated successfully. Varying the waveform from sine-wave to square-wave allows generating a fluid convection at specific positions to mix the antibody-functionalized beads and antigen. Potential application in the bead-based immunoassay was also demonstrated for immuno-reaction and subsequently separating the bead-bead aggregate and non-binding beads on-chip.

原文English
文章編號066501
期刊Biomicrofluidics
8
發行號6
DOIs
出版狀態Published - 2014 十二月 30

指紋

Electroosmosis
Convection
Microfluidics
electrokinetics
ripples
Immunoassay
Particle Size
beads
Adhesives
chips
Antigens
Glues
Antibodies
Electrophoresis
Electric fields
Microstructure
Fluids
immunoassay
glues
square waves

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

引用此文

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Ripple structure-generated hybrid electrokinetics for on-chip mixing and separating of functionalized beads. / Cheng, I. Fang; Chiang, Sheng Chuan; Chung, Cheng Che; Yeh, Trai-Ming; Chang, Hsien-Chang.

於: Biomicrofluidics, 卷 8, 編號 6, 066501, 30.12.2014.

研究成果: Article

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AU - Cheng, I. Fang

AU - Chiang, Sheng Chuan

AU - Chung, Cheng Che

AU - Yeh, Trai-Ming

AU - Chang, Hsien-Chang

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