ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury

Kensuke Noma, Yoshiyuki Rikitake, Naotsugu Oyama, Guijun Yan, Pilar Alcaide, Ping Yen Liu, Hongwei Wang, Daniela Ahl, Naoki Sawada, Ryuji Okamoto, Yukio Hiroi, Koichi Shimizu, Francis W. Luscinskas, Jianxin Sun, James K. Liao

研究成果: Article同行評審

147 引文 斯高帕斯(Scopus)

摘要

Although Rho-associated kinase (ROCK) activity has been implicated in cardiovascular diseases, the tissue- and isoform-specific roles of ROCKs in the vascular response to injury are not known. To address the role of ROCKs in this process, we generated haploinsufficient Rock1 (Rock1+/-) and Rock2 (Rock2+/-) mice and performed carotid artery ligations. Following this intervention, we found reduced neointima formation in Rock1+/- mice compared with that of WT or Rock2+/- mice. This correlated with decreased vascular smooth muscle cell proliferation and survival, decreased levels proinflammatory adhesion molecule expression, and reduced leukocyte infiltration. In addition, thioglycollate-induced peritoneal leukocyte recruitment and accumulation were substantially reduced in Rock1+/- mice compared with those of WT and Rock2+/- mice. To determine the role of leukocyte-derived ROCK1 in neointima formation, we performed reciprocal bone marrow transplantation (BMT) in WT and Rock1+/- mice. Rock1 +/- to WT BMT led to reduced neointima formation and leukocyte infiltration following carotid ligation compared with those of WT to WT BMT. In contrast, WT to Rock1+/- BMT resulted in increased neointima formation. These findings indicate that ROCK1 in BM-derived cells mediates neointima formation following vascular injury and suggest that ROCK1 may represent a promising therapeutic target in vascular inflammatory diseases.

原文English
頁(從 - 到)1632-1644
頁數13
期刊Journal of Clinical Investigation
118
發行號5
DOIs
出版狀態Published - 2008 5月 1

All Science Journal Classification (ASJC) codes

  • 一般醫學

指紋

深入研究「ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury」主題。共同形成了獨特的指紋。

引用此