TY - JOUR
T1 - Role of apoptosis in growth and differentiation of proximal tubule cells in primary cultures
AU - Tang, Ming Jer
AU - Cheng, Ya Ren
AU - Lin, Hsi Hui
N1 - Funding Information:
The authors thank Dr. Paul W-F Poon for critical reading and suggestion of the manuscript. This work was supported by NSC Grant 83-0412-B006-100 to M.-J.T.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/1/26
Y1 - 1996/1/26
N2 - In this study, we assessed the profile of cell proliferation, Na,K-ATPase activity and apoptosis in primary cultures of proximal tubule cells. Freshly isolated proximal tubules exhibited apoptosis very early in culture: floated cells within 6 hr; attached tubule cells within 20 hr. The onset of apoptosis in either attached or floated proximal tubules was accompanied by a decline of Na,K-ATPase activity, indicating a close relationship between apoptosis and dedifferentiation. The ratio of apoptosis was highest at the lag phase and lowest at the rapid growth phase, reflecting an inverse relationship between mitogenesis and apoptosis. Our data indicate that apoptosis is involved in the growth and early downregulation of Na,K-ATPase activity in primary cultures of proximal tubule cells.
AB - In this study, we assessed the profile of cell proliferation, Na,K-ATPase activity and apoptosis in primary cultures of proximal tubule cells. Freshly isolated proximal tubules exhibited apoptosis very early in culture: floated cells within 6 hr; attached tubule cells within 20 hr. The onset of apoptosis in either attached or floated proximal tubules was accompanied by a decline of Na,K-ATPase activity, indicating a close relationship between apoptosis and dedifferentiation. The ratio of apoptosis was highest at the lag phase and lowest at the rapid growth phase, reflecting an inverse relationship between mitogenesis and apoptosis. Our data indicate that apoptosis is involved in the growth and early downregulation of Na,K-ATPase activity in primary cultures of proximal tubule cells.
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U2 - 10.1006/bbrc.1996.0118
DO - 10.1006/bbrc.1996.0118
M3 - Article
C2 - 8579570
AN - SCOPUS:0030053799
SN - 0006-291X
VL - 218
SP - 658
EP - 664
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -