Role of ciliary neurotrophic factor in microglial phagocytosis

Tsung I. Lee, Chung Shi Yang, Kuan Ming Fang, Shun Fen Tzeng

研究成果: Article同行評審

18 引文 斯高帕斯(Scopus)

摘要

Microglia, CNS-resident macrophages, serve as scavengers to remove cellular debris and facilitate tissue remodeling in the developing and injured CNS. Little is known as what and how microenvironmental factors mediate the phagocytotic ability of microglia. Our previous study has indicated that treatment with glial cell line-derived neurotrophic factor (GDNF) increased the phagocytotic activity of primary rat microglia possibly through the upregulation of α5 integrin. In the present study, ciliary neurotrophic factor (CNTF), which has been reported to be produced by glia, was shown to have stimulatory effect on the phagocytosis of primary rat microglia and mouse microglial cell line BV2. Ca2+ imaging analysis and the application of intracellular calcium chelator BAPTA-AM revealed that CNTF-induced increase in microglial phagocytosis was mediated by a calcium signaling pathway. Furthermore, treatment with CNTF led to an increase in the expression of αv integrin, which has been reported to be involved in the phagocytosis of the apoptotic cells. In summary, we have provided evidence that CNTF can increase microglial phagocytosis through a calcium-mediated pathway. Our results also suggest that the upregulation of αv integrin by CNTF could be involved in the increased phagocytotic activity of microglia.

原文English
頁(從 - 到)109-117
頁數9
期刊Neurochemical Research
34
發行號1
DOIs
出版狀態Published - 2009 一月

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 細胞與分子神經科學

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