Role of JNK activation in paclitaxel-induced apoptosis in human head and neck squamous cell carcinoma

YU YAN LAN, YING HUI CHEN, CHENG LIU, KUO LUNG TUNG, YEN TING WU, SHENG CHIEH LIN, CHIN HAN WU, HONG YI CHANG, YUNG CHIA CHEN, BU MIIN HUANG

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

It has been reported that paclitaxel activates cell cycle arrest and increases caspase protein expression to induce apoptosis in head and neck squamous cell carcinoma (HNSCC) cell lines. However, the potential signaling pathway regulating this apoptotic phenomenon remains unclear. The present study used OEC-M1 cells to investigate the underlying molecular mechanism of paclitaxel-induced apoptosis. Following treatment with paclitaxel, cell viability was assessed via the MTT assay. Necrosis, apoptosis, cell cycle and mitochondrial membrane potential (ΔΨm) were analyzed via flow cytometric analyses, respectively. Western blot analysis was performed to detect the expression levels of proteins associated with the MAPK and caspase signaling pathways. The results demonstrated that low-dose paclitaxel (50 nM) induced apoptosis but not necrosis in HNSCC cells. In addition, paclitaxel activated the c-Jun N-terminal kinase (JNK), but not extracellular signal-regulated kinase or p38 mitogen-activated protein kinase. The paclitaxel-activated JNK contributed to paclitaxel-induced apoptosis, activation of caspase-3, -6, -7, -8 and -9, and reduction of ΔΨm. In addition, caspase-8 and -9 inhibitors, respectively, significantly decreased paclitaxel-induced apoptosis. Notably, Bid was truncated following treatment with paclitaxel. Taken together, the results of the present study suggest that paclitaxel-activated JNK is required for caspase activation and loss of ΔΨm, which results in apoptosis of HNSCC cells. These results may provide mechanistic basis for designing more effective paclitaxel-combining regimens to treat HNSCC.

原文English
文章編號A10
期刊Oncology Letters
22
發行號4
DOIs
出版狀態Published - 2021 十月

All Science Journal Classification (ASJC) codes

  • 腫瘤科
  • 癌症研究

指紋

深入研究「Role of JNK activation in paclitaxel-induced apoptosis in human head and neck squamous cell carcinoma」主題。共同形成了獨特的指紋。

引用此