Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans

Jia Horung Hung, Zhao Xiang Wang, Yuan Hsin Lo, Chaw Ning Lee, Yun Chang, Rita Yuchiao Chang, Chien Chun Huang, Tak Wah Wong

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

Invasive Candida albicans infection is a significant opportunistic fungal infection in humans because it is one of the most common colonizers of the gut, mouth, vagina, and skin. Despite the availability of antifungal medication, the mortality rate of invasive candidiasis remains ~50%. Unfortunately, the incidence of drug-resistant C. albicans is increasing globally. Antimicrobial photodynamic therapy (aPDT) may offer an alternative or adjuvant treatment to inhibit C. albicans biofilm formation and overcome drug resistance. Rose bengal (RB)-mediated aPDT has shown effective cell killing of bacteria and C. albicans. In this study, the efficacy of RB-aPDT on multidrugresistant C. albicans is described. A homemade green light-emitting diode (LED) light source is designed to align with the center of a well of a 96-well plate. The yeasts were incubated in the wells with different concentrations of RB and illuminated with varying fluences of green light. The killing effects were analyzed by the plate dilution method. With an optimal combination of light and RB, 3-log growth inhibition was achieved. It was concluded that RB-aPDT might potentially inhibit drug-resistant C. albicans.

原文English
文章編號e63558
期刊Journal of Visualized Experiments
2022
發行號181
DOIs
出版狀態Published - 2022 3月

All Science Journal Classification (ASJC) codes

  • 神經科學 (全部)
  • 化學工程 (全部)
  • 生物化學、遺傳與分子生物學 (全部)
  • 免疫學與微生物學 (全部)

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