Rosmanol potently inhibits lipopolysaccharide-induced iNOS and COX-2 expression through downregulating MAPK, NF-κB, STAT3 and C/EBP signaling pathways

Ching Shu Lai, Jong Hun Lee, Chi Tang Ho, Cheng Bin Liu, Ju-Ming Wang, Ying-Jan Wang, Min Hsiung Pan

研究成果: Article

68 引文 (Scopus)

摘要

Rosmanol is a natural polyphenol from the herb rosemary (Rosmarinus officinalis L.) with high antioxidant activity. In this study, we investigated the inhibitory effects of rosmanol on the induction of NO synthase (NOS) and COX-2 in RAW 264.7 cells induced by lipopolysaccharide (LPS). Rosmanol markedly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression, as well as the downstream products, NO and PGE2. Treatment with rosmanol also reduced translocation of the nuclear factor-κB (NF-κB) subunits by prevention of the degradation and phosphorylation of inhibitor κB (IκB). Western blot analysis showed that rosmanol significantly inhibited translocation and phosphorylation of NF-κB, signal transducer and activator of transcription-3 (STAT3), and the protein expression of C/EBPβ and C/EBPδ. We also found that rosmanol suppressed LPS-induced phosphorylation of ERK1/2, p38 mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling. Our results demonstrate that rosmanol downregulates inflammatory iNOS and COX-2 gene expression by inhibiting the activation of NF-κB and STAT3 through interfering with the activation of PI3K/Akt and MAPK signaling. Taken together, rosmanol might contribute to the potent anti-inflammatory effect of rosemary and may have potential to be developed into an effective anti-inflammatory agent.

原文English
頁(從 - 到)10990-10998
頁數9
期刊Journal of Agricultural and Food Chemistry
57
發行號22
DOIs
出版狀態Published - 2009 十一月 25

指紋

STAT3 Transcription Factor
Complement Factor B
prostaglandin synthase
Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
lipopolysaccharides
Lipopolysaccharides
phosphorylation
Down-Regulation
transcription (genetics)
rosemary
phosphatidylinositol 3-kinase
protein synthesis
Phosphorylation
anti-inflammatory agents
gene expression
Rosmarinus officinalis
Phosphatidylinositol 3-Kinase
anti-inflammatory activity
herbs

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

引用此文

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title = "Rosmanol potently inhibits lipopolysaccharide-induced iNOS and COX-2 expression through downregulating MAPK, NF-κB, STAT3 and C/EBP signaling pathways",
abstract = "Rosmanol is a natural polyphenol from the herb rosemary (Rosmarinus officinalis L.) with high antioxidant activity. In this study, we investigated the inhibitory effects of rosmanol on the induction of NO synthase (NOS) and COX-2 in RAW 264.7 cells induced by lipopolysaccharide (LPS). Rosmanol markedly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression, as well as the downstream products, NO and PGE2. Treatment with rosmanol also reduced translocation of the nuclear factor-κB (NF-κB) subunits by prevention of the degradation and phosphorylation of inhibitor κB (IκB). Western blot analysis showed that rosmanol significantly inhibited translocation and phosphorylation of NF-κB, signal transducer and activator of transcription-3 (STAT3), and the protein expression of C/EBPβ and C/EBPδ. We also found that rosmanol suppressed LPS-induced phosphorylation of ERK1/2, p38 mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling. Our results demonstrate that rosmanol downregulates inflammatory iNOS and COX-2 gene expression by inhibiting the activation of NF-κB and STAT3 through interfering with the activation of PI3K/Akt and MAPK signaling. Taken together, rosmanol might contribute to the potent anti-inflammatory effect of rosemary and may have potential to be developed into an effective anti-inflammatory agent.",
author = "Lai, {Ching Shu} and Lee, {Jong Hun} and Ho, {Chi Tang} and Liu, {Cheng Bin} and Ju-Ming Wang and Ying-Jan Wang and Pan, {Min Hsiung}",
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Rosmanol potently inhibits lipopolysaccharide-induced iNOS and COX-2 expression through downregulating MAPK, NF-κB, STAT3 and C/EBP signaling pathways. / Lai, Ching Shu; Lee, Jong Hun; Ho, Chi Tang; Liu, Cheng Bin; Wang, Ju-Ming; Wang, Ying-Jan; Pan, Min Hsiung.

於: Journal of Agricultural and Food Chemistry, 卷 57, 編號 22, 25.11.2009, p. 10990-10998.

研究成果: Article

TY - JOUR

T1 - Rosmanol potently inhibits lipopolysaccharide-induced iNOS and COX-2 expression through downregulating MAPK, NF-κB, STAT3 and C/EBP signaling pathways

AU - Lai, Ching Shu

AU - Lee, Jong Hun

AU - Ho, Chi Tang

AU - Liu, Cheng Bin

AU - Wang, Ju-Ming

AU - Wang, Ying-Jan

AU - Pan, Min Hsiung

PY - 2009/11/25

Y1 - 2009/11/25

N2 - Rosmanol is a natural polyphenol from the herb rosemary (Rosmarinus officinalis L.) with high antioxidant activity. In this study, we investigated the inhibitory effects of rosmanol on the induction of NO synthase (NOS) and COX-2 in RAW 264.7 cells induced by lipopolysaccharide (LPS). Rosmanol markedly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression, as well as the downstream products, NO and PGE2. Treatment with rosmanol also reduced translocation of the nuclear factor-κB (NF-κB) subunits by prevention of the degradation and phosphorylation of inhibitor κB (IκB). Western blot analysis showed that rosmanol significantly inhibited translocation and phosphorylation of NF-κB, signal transducer and activator of transcription-3 (STAT3), and the protein expression of C/EBPβ and C/EBPδ. We also found that rosmanol suppressed LPS-induced phosphorylation of ERK1/2, p38 mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling. Our results demonstrate that rosmanol downregulates inflammatory iNOS and COX-2 gene expression by inhibiting the activation of NF-κB and STAT3 through interfering with the activation of PI3K/Akt and MAPK signaling. Taken together, rosmanol might contribute to the potent anti-inflammatory effect of rosemary and may have potential to be developed into an effective anti-inflammatory agent.

AB - Rosmanol is a natural polyphenol from the herb rosemary (Rosmarinus officinalis L.) with high antioxidant activity. In this study, we investigated the inhibitory effects of rosmanol on the induction of NO synthase (NOS) and COX-2 in RAW 264.7 cells induced by lipopolysaccharide (LPS). Rosmanol markedly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression, as well as the downstream products, NO and PGE2. Treatment with rosmanol also reduced translocation of the nuclear factor-κB (NF-κB) subunits by prevention of the degradation and phosphorylation of inhibitor κB (IκB). Western blot analysis showed that rosmanol significantly inhibited translocation and phosphorylation of NF-κB, signal transducer and activator of transcription-3 (STAT3), and the protein expression of C/EBPβ and C/EBPδ. We also found that rosmanol suppressed LPS-induced phosphorylation of ERK1/2, p38 mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling. Our results demonstrate that rosmanol downregulates inflammatory iNOS and COX-2 gene expression by inhibiting the activation of NF-κB and STAT3 through interfering with the activation of PI3K/Akt and MAPK signaling. Taken together, rosmanol might contribute to the potent anti-inflammatory effect of rosemary and may have potential to be developed into an effective anti-inflammatory agent.

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