Salinity-driven nitrogen removal and its quantitative molecular mechanisms in artificial tidal wetlands

Manping Zhang, Zi Jing Wang, Jung Chen Huang, Shanshan Sun, Xijun Cui, Weili Zhou, Shengbing He

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)


The present study investigated the performance in nitrogen removal and associated nitrogen transformation processes in seven mesocosms fed with saline water (0‰ to 30‰) to simulate tidal flow constructed wetlands (TF CWs). The highly effective and steady removal of NH4+-N (84.74% averagely) was obtained at various salinities, while the rates varied from 6.34% to 89.19% and 22.54% to 87.48% for NO3-N and total nitrogen (TN), respectively. Overall, nitrogen removal efficiencies were greater at lower salinities. Molecular biological analyses verified the co-occurrence of dissimilatory nitrate reduction to ammonium (DNRA), denitrification, anaerobic ammonium oxidation (anammox) and nitrification in the mesocosms, reportedly contributing to nitrogen removal in TF CWs. The absolute copy numbers of nitrogen functional genes and total bacterial 16S rRNA were 2.54 × 103-7.35 × 107 and 3.21 × 107-7.82 × 109 copies g−1 dg (dry gravel), respectively, with the dominant phyla, i.e., Chloroflexi, Proteobacteria, Actinobacteriota, Cyanobacteria, and Firmicutes, accounting for over 80% of the sequences. The relative abundances of the genera related to nitrification and dissimilatory nitrate reduction processes, i.e., denitrification, anammox and DNRA, varied from 0.16% to 0.89% and from 3.66% to 11.59%, respectively, while quantitative relationships confirmed NH4+-N transformation rate was jointly controlled by amoA, hzsB, nxrA and nrfA, and NO3-N removal rate by nirS, nosZ, narG, qnorB and nxrA. These findings may shed light on quantitative molecular mechanisms for nitrogen removal in TF CWs for the saline water treatment, providing a sustainable solution to nitrogen pollution problem in the estuary ecosystem.

期刊Water Research
出版狀態Published - 2021 9月 1

All Science Journal Classification (ASJC) codes

  • 環境工程
  • 土木與結構工程
  • 生態建模
  • 水科學與技術
  • 廢物管理和處置
  • 污染


深入研究「Salinity-driven nitrogen removal and its quantitative molecular mechanisms in artificial tidal wetlands」主題。共同形成了獨特的指紋。