In the era of knowledge economy, learning has become increasingly important, it is more and more convenient to acquire the information in a fast way and E-learning has become increasingly popular. Under this trend, the traditional teaching method in the classroom will not be the only choice any longer and the application of E-learning system will be the important trend in the future. In recent years, related governmental departments also vigorously promote the E-learning system. Some special courses enable learners to firstly possess the prior knowledge of agriculture and then the relevant agricultural knowledge is digitalized; lastly, the professional teachers will be equipped for teaching and a system platform for high-efficient learning will be provided through E-learning. The learning effectiveness desired by this research through the establishment of an agricultural E-learning platform is shown as below: (1) apply E-learning platform to increase learning variety, further enhance learners’ knowledge-ability and learning willingness, break through the territory restriction in learning and facilitate the learning; (2) apply satisfaction survey and assessment mechanism to understand the learning effectiveness of E-learning platform. This research established the agricultural E-learning platform and used students’ satisfaction with information contents and course contents for E-learning system obtained through the satisfaction survey to present the positive affirmation. Therefore, it can be known that E-learning is greatly helpful to improve agricultural workers’ learning.