Selective inhibition of early-but not late-expressed HIF-1α is neuroprotective in rats after focal ischemic brain damage

Shiu Hwa Yeh, Li Chin Ou, Po-Wu Gean, Jan-Jong Hung, Wen Chang Chang

研究成果: Article同行評審

64 引文 斯高帕斯(Scopus)

摘要

The expression of hypoxia-inducible factor-1-alpha (HIF-1α) is upregulated in ischemic stroke, but its function is still unclear. In the present study, biphasic expression of HIF-1α was observed during 1-12 h and after 48 h in neurons exposed to ischemic stress in vitro and in vivo. Treating neurons with 2-methoxyestradiol (2ME2) 0.5 h after ischemic stress or pre-silencing HIF-1α with small interfering RNA (siRNA) decreased brain injury, brain edema and number of apoptotic cell, and downregulates Nip-like protein X (Nix) expression. Conversely, applying 2ME2 to neurons 8 h after ischemic stress or silencing the HIF-1α with siRNA 12 h after oxygen-glucose deprivation (OGD) increased neuron damage and decreased vascular endothelial growth factor (VEGF) expression. Taken together, these results demonstrate that HIF-1α induced by ischemia in early and late times leads cellular apoptosis and survival, respectively, and provides a new insight into the divergent roles of HIF-1α expression in neurons after ischemic stroke.

原文English
頁(從 - 到)249-262
頁數14
期刊Brain Pathology
21
發行號3
DOIs
出版狀態Published - 2011 五月 1

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

指紋 深入研究「Selective inhibition of early-but not late-expressed HIF-1α is neuroprotective in rats after focal ischemic brain damage」主題。共同形成了獨特的指紋。

引用此