Self-assembly DNA polyplex vaccine inside dissolving microneedles for high-potency intradermal vaccination

Jing Fong Liao, Jin Ching Lee, Chun Kuang Lin, Kuo Chen Wei, Pin Yuan Chen, Hung Wei Yang

研究成果: Article同行評審

42 引文 斯高帕斯(Scopus)


The strong immunogenicity induction is the powerful weapon to prevent the virus infections. This study demonstrated that one-step synthesis of DNA polyplex vaccine in microneedle (MN) patches can induce high immunogenicity through intradermal vaccination and increase the vaccine stability for storage outside the cold chain. More negative charged DNA vaccine was entrapped into the needle region of MNs followed by DNA polyplex formation with branched polyethylenimine (bPEI) pre-coated in the cavities of polydimethylsiloxane (PDMS) molds that can deliver more DNA vaccine to immune-cell rich epidermis with high transfection efficiency. Our data in this study support the safety and immunogenicity of the MN-based vaccine; the MN patch delivery system induced an immune response 3.5-fold as strong as seen with conventional intramuscular administration; the DNA polyplex formulation provided excellent vaccine stability at high temperature (could be stored at 45°C for at least 4 months); the DNA vaccine is expected to be manufactured at low cost and not generate sharps waste. We think this study is significant to public health because there is a pressing need for an effective vaccination in developing countries.

頁(從 - 到)2593-2605
出版狀態Published - 2017

All Science Journal Classification (ASJC) codes

  • 醫藥(雜項)
  • 藥理學、毒理學和藥劑學(雜項)


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