Sequence and expression of testis-expressed gene 14 (Tex14): A gene encoding a protein kinase preferentially expressed during spermatogenesis

Meng Hsing Wu, Aleksandar Rajkovic, Kathleen H. Burns, Wei Yan, Yi Nan Lin, Martin M. Matzuk

研究成果: Article同行評審

33 引文 斯高帕斯(Scopus)

摘要

To discover germ cell-specific genes, we used in silico subtraction and identified testis expressed gene 14 (Tex14). Mouse Tex14 contains an open reading frame encoding a 1450-amino-acid protein, which shares 64% amino acid identity with the predicted human TEX14 protein. The predicted TEX14 amino acid sequence consists of three ankyrin repeats, a protein kinase domain, and a leucine zipper dimerization motif. Northern blot analysis and in situ hybridization show that Tex14 mRNA is expressed specifically in the testis, with highest levels observed in pachytene, diplotene, and meiotically dividing spermatocytes. Two 5′ splice variants of mouse Tex14 were discovered by sequencing 5′-RACE polymerase chain reaction products. TEX14 is predicted to be localized to the nucleus, suggesting that it may play a key role in regulating gene expression or modulating nuclear events during mammalian spermatogenesis.

原文English
頁(從 - 到)231-236
頁數6
期刊Gene Expression Patterns
3
發行號2
DOIs
出版狀態Published - 2003 五月

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 遺傳學
  • 發展生物學

指紋

深入研究「Sequence and expression of testis-expressed gene 14 (Tex14): A gene encoding a protein kinase preferentially expressed during spermatogenesis」主題。共同形成了獨特的指紋。

引用此