Short-term temporal metabolic behavior in halophilic cyanobacterium synechococcus sp. Strain PCC 7002 after salt shock

Shimpei Aikawa, Atsumi Nishida, Tomohisa Hasunuma, Jo Shu Chang, Akihiko Kondo

研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)

摘要

In response to salt stress, cyanobacteria increases the gene expression of Na+/H+ antiporter and K+ uptake system proteins and subsequently accumulate compatible solutes. However, alterations in the concentrations of metabolic intermediates functionally related to the early stage of the salt stress response have not been investigated. The halophilic cyanobacterium Synechococcus sp. PCC 7002 was subjected to salt shock with 0.5 and 1 M NaCl, then we performed metabolomics analysis by capillary electrophoresis/mass spectrometry (CE/MS) and gas chromatography/mass spectrometry (GC/MS) after cultivation for 1, 3, 10, and 24 h. Gene expression profiling using a microarray after 1 h of salt shock was also conducted. We observed suppression of the Calvin cycle and activation of glycolysis at both NaCl concentrations. However, there were several differences in the metabolic changes after salt shock following exposure to 0.5 M and 1 M NaCl: (i): the main compatible solute, glucosylglycerol, accumulated quickly at 0.5 M NaCl after 1 h but increased gradually for 10 h at 1 M NaCl; (ii) the oxidative pentose phosphate pathway and the tricarboxylic acid cycle were activated at 0.5 M NaCl; and (iii) the multi-functional compound spermidine greatly accumulated at 1 M NaCl. Our results show that Synechococcus sp. PCC 7002 acclimated to different levels of salt through a salt stress response involving the activation of different metabolic pathways.

原文English
文章編號297
期刊Metabolites
9
發行號12
DOIs
出版狀態Published - 2019 12月

All Science Journal Classification (ASJC) codes

  • 內分泌學、糖尿病和代謝
  • 生物化學
  • 分子生物學

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