Simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes with an array-based immunosorbent assay using universal protein G-liposomal nanovesicles

Chien-Sheng Chen, Richard A. Durst

研究成果: Article

57 引文 斯高帕斯(Scopus)

摘要

A novel universal reagent for immunoassays, protein G-liposomal nanovesicles has been developed and successfully used in an immunomagnetic bead sandwich assay for the detection of Escherichia coli O157:H7 [C.-S. Chen, A.J. Baeumner, R.A. Durst, Talanta 67 (2005) 205]. To demonstrate the universal capability of protein G-liposomal nanovesicles, this reagent was used to develop an array-based immunosorbent assay for the simultaneous detection of E. coli O157:H7, Salmonella, and Listeria monocytogenes. Both direct and competitive immunoassay formats were used to demonstrate the feasibility of detecting multiple analytes in a single test by using universal protein G-liposomal nanovesicles. Both pure and mixed cultures were examined in the direct immunoassay format. Results indicate that the limits of detection (LODs) of the direct assay for E. coli O157:H7, Salmonella enterica serovar Typhimurium and L. monocytogenes in pure cultures were approximately 100, 500 and 1.5 × 104 CFU/ml, respectively. In mixed cultures, the LODs were approximately 3.1 × 103, 7.8 × 104, and 7.9 × 105 CFU/ml. In the competitive assay format, the LODs for E. coli O157:H7, S. enterica serovar Typhimurium, and L. monocytogenes were approximately 1.5 × 104, 5 × 104, and 1.2 × 105 CFU/ml for the pure cultures. These results showed that protein G-liposomal nanovesicles can be successfully used in a simultaneous immunoassay for several food-borne pathogens, thereby demonstrating that they are effective universal reagents for use in immunoassays.

原文English
頁(從 - 到)232-238
頁數7
期刊Talanta
69
發行號1
DOIs
出版狀態Published - 2006 三月 15

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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