@article{3d1f92165d704b299bdebac45e361f68,
title = "Single-cell RNA sequencing uncovers the individual alteration of intestinal mucosal immunocytes in Dusp6 knockout mice",
abstract = "Single-cell RNA sequencing (scRNA-seq) approach can broadly and specifically evaluate the individual cells with minimum detection bias. To explore the individual compositional and transcriptional alteration of intestinal leukocytes in the Dual Specificity Phosphatase six knockout (D6KO) mice, we performed a scRNA-seq followed by the cell type annotation based on ImmGen database. Composition assessments found that D6KO-derived intestinal leukocytes tend to stay inactivate or immature status. The enrichment analysis showed that D6KO-derived intestinal leukocytes are less sensitive to microbes. The mod PhEA phenotypic analysis showed that the D6KO leukocytes may link to not only immune-associated but also diverse previously non-immune-related diseases. Integrating our dataset with the published dataset GSE124880 generated a comprehensive dataset for exploring intestinal immunity. Down-regulation of Ccl17 gene was found in the D6KO-derived dendritic cells. Our results demonstrated the advantage of applying scRNA-seq for dissecting the individual alteration of intestinal leukocytes, particularly in the D6KO mice at a naive state.",
author = "Chang, {Cherng Shyang} and Yu, {Wen Hsuan} and Su, {Chang Chao} and Ruan, {Jhen Wei} and Lin, {Chiao Mei} and Huang, {Chih Ting} and Tsai, {Yi Ting} and Lin, {I. Jung} and Lai, {Chao Yang} and Chuang, {Tsung Hsien} and Lin, {Wen Jye} and Tan, {Tse Hua} and Huang, {Hsuan Cheng} and Juan, {Hsueh Fen} and Kao, {Cheng Yuan}",
note = "Funding Information: We are grateful to Nir Hacohen at Broad Institute of MIT and Harvard for the suggestions on the experimental design of scRNA-seq. We thank Chao-Yang Hsiao at the Core Instrument Center in National Health Research Institutes (NHRI) for assistance of FACS experiment and the technical support for scRNA-seq platform by BIOTOOLS Co., Ltd. This work was supported by the following grants: IM-108-PP-04 from NHRI , Taiwan; CYC109003 from National Cheng Kung University , Taiwan and Ditmanson Medical Foundation Chia-Yi Christian Hospital , Taiwan; and 106-2628-B-400-001-MY3 , 106-2923-B-400-001-MY3 , 108-2321-B-400-011- , 109-2221-E-010-012-MY3 , 109-2327-B-400-001- , 109-2320-B-400-008-MY3 , 109-2221-E-002-161-MY3 , 109-2221-E-010-012-MY3 from MOST , Taiwan. Funding Information: We are grateful to Nir Hacohen at Broad Institute of MIT and Harvard for the suggestions on the experimental design of scRNA-seq. We thank Chao-Yang Hsiao at the Core Instrument Center in National Health Research Institutes (NHRI) for assistance of FACS experiment and the technical support for scRNA-seq platform by BIOTOOLS Co. Ltd. This work was supported by the following grants: IM-108-PP-04 from NHRI, Taiwan; CYC109003 from National Cheng Kung University, Taiwan and Ditmanson Medical Foundation Chia-Yi Christian Hospital, Taiwan; and 106-2628-B-400-001-MY3, 106-2923-B-400-001-MY3, 108-2321-B-400-011-, 109-2221-E-010-012-MY3, 109-2327-B-400-001-, 109-2320-B-400-008-MY3, 109-2221-E-002-161-MY3, 109-2221-E-010-012-MY3 from MOST, Taiwan. C.-S.C. W.-H.Y. C.-C.S. J.-W. R. H.-C.H. H.-F.J. and C.-Y.K. designed the study and interpreted the data. C.-S.C. J.-W.R. C.-T.H. C.-M.L. Y.-T.T. and C.-Y.L. performed animal and isolation experiments. W.-H.Y. I.-J.L. H.-C.H. and H.-F.J. performed computational analysis. T.-H.T. provided D6KO mice. C.-S.C. and W.-H.Y. wrote the manuscript. J.-W.R. C.-C.S. T.-H.C. W.-J.L. H.-C.H. H.-F.J. and C.-Y.K. edited the manuscript. C.-Y.K. performed overall supervision of the project. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = feb,
day = "18",
doi = "10.1016/j.isci.2022.103738",
language = "English",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "2",
}