Single-cell RNA sequencing uncovers the individual alteration of intestinal mucosal immunocytes in Dusp6 knockout mice

Cherng Shyang Chang, Wen Hsuan Yu, Chang Chao Su, Jhen Wei Ruan, Chiao Mei Lin, Chih Ting Huang, Yi Ting Tsai, I. Jung Lin, Chao Yang Lai, Tsung Hsien Chuang, Wen Jye Lin, Tse Hua Tan, Hsuan Cheng Huang, Hsueh Fen Juan, Cheng Yuan Kao

研究成果: Article同行評審

摘要

Single-cell RNA sequencing (scRNA-seq) approach can broadly and specifically evaluate the individual cells with minimum detection bias. To explore the individual compositional and transcriptional alteration of intestinal leukocytes in the Dual Specificity Phosphatase six knockout (D6KO) mice, we performed a scRNA-seq followed by the cell type annotation based on ImmGen database. Composition assessments found that D6KO-derived intestinal leukocytes tend to stay inactivate or immature status. The enrichment analysis showed that D6KO-derived intestinal leukocytes are less sensitive to microbes. The mod PhEA phenotypic analysis showed that the D6KO leukocytes may link to not only immune-associated but also diverse previously non-immune-related diseases. Integrating our dataset with the published dataset GSE124880 generated a comprehensive dataset for exploring intestinal immunity. Down-regulation of Ccl17 gene was found in the D6KO-derived dendritic cells. Our results demonstrated the advantage of applying scRNA-seq for dissecting the individual alteration of intestinal leukocytes, particularly in the D6KO mice at a naive state.

原文English
文章編號103738
期刊iScience
25
發行號2
DOIs
出版狀態Published - 2022 2月 18

All Science Journal Classification (ASJC) codes

  • 多學科

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