TY - JOUR
T1 - Single pulsed electromagnetic field restores bone mass and microarchitecture in denervation/disuse osteopenic mice
AU - Lin, Chih Chun
AU - Chang, Yu Ting
AU - Lin, Ru Wei
AU - Chang, Chih Wei
AU - Wang, Gwo Jaw
AU - Lai, Kuo An
N1 - Funding Information:
The animal use protocol was reviewed and approved by the National Cheng Kung University Institutional Animal Care and Use Committee. This study is in memory of Professor Kao-Chi Chung, who devoted himself to the invention and development of SPEMF. We are also grateful to Professor Tsung-Hsueh Lu and Professor Chung-Yi Li for providing statistical consulting services from the Biostatistics Consulting Center, National Cheng Kung University Hospital. This study was supported in part by Ministry of Science and Technology of Taiwan (Grant number: MOST 103-2314-B-006-032) and the Medical Device Innovation Center (MDIC), National Cheng Kung University (NCKU) from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MoE) in Taiwan.
Funding Information:
This study was supported in part by Ministry of Science and Technology of Taiwan (Grant number: MOST 103-2314-B-006-032 ) and the Medical Device Innovation Center (MDIC), National Cheng Kung University (NCKU) from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MoE) in Taiwan.
Publisher Copyright:
© 2019
PY - 2020/6
Y1 - 2020/6
N2 - Pulsed electromagnetic fields (PEMFs) have been proposed to treat bone loss. However, time-consuming is the main complaint. A time-saving and effective treatment is of expectation. Previously, we showed a 3 min daily of single pulsed electromagnetic field (SPEMF) accelerated bone formation of long bone defect in mice. Here we compared the effect of SPEMF with PEMF for treating denervation/disuse osteopenic mice. Healthy mice were divided into 3 groups: intact mice (INT), INT + PEMF, and INT + SPEMF. Induced osteopenic mice were divided to osteopenia (IOP), IOP + PEMF, and IOP + SPEMF groups. The PEMF treated groups were subjected to daily 8 h PEMF(15 Hz, 18 G) exposure, while SPEMF treated groups were daily 3 min SPEMF(0.2 Hz, 1 T) exposure. BMD was evaluated every two weeks during the 12 weeks of treatment. Microarchitecture was evaluated on week 12. SPEMF significantly reversed bone loss in IOP mice as early as 6 weeks post-treatment, while PEMF reversed bone loss after 8 weeks. Bone volume was significantly increased in the IOP + PEMF and IOP + SPEMF group. Besides, bone volume and trabecular number of IOP + SPEMF mice were restored to the levels of INT mice in 12 weeks. Our finding suggests SPEMF increased BMD and restored microarchitecture of disuse osteopenic mice to healthy level.
AB - Pulsed electromagnetic fields (PEMFs) have been proposed to treat bone loss. However, time-consuming is the main complaint. A time-saving and effective treatment is of expectation. Previously, we showed a 3 min daily of single pulsed electromagnetic field (SPEMF) accelerated bone formation of long bone defect in mice. Here we compared the effect of SPEMF with PEMF for treating denervation/disuse osteopenic mice. Healthy mice were divided into 3 groups: intact mice (INT), INT + PEMF, and INT + SPEMF. Induced osteopenic mice were divided to osteopenia (IOP), IOP + PEMF, and IOP + SPEMF groups. The PEMF treated groups were subjected to daily 8 h PEMF(15 Hz, 18 G) exposure, while SPEMF treated groups were daily 3 min SPEMF(0.2 Hz, 1 T) exposure. BMD was evaluated every two weeks during the 12 weeks of treatment. Microarchitecture was evaluated on week 12. SPEMF significantly reversed bone loss in IOP mice as early as 6 weeks post-treatment, while PEMF reversed bone loss after 8 weeks. Bone volume was significantly increased in the IOP + PEMF and IOP + SPEMF group. Besides, bone volume and trabecular number of IOP + SPEMF mice were restored to the levels of INT mice in 12 weeks. Our finding suggests SPEMF increased BMD and restored microarchitecture of disuse osteopenic mice to healthy level.
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U2 - 10.1016/j.medengphy.2019.10.004
DO - 10.1016/j.medengphy.2019.10.004
M3 - Article
C2 - 32376078
AN - SCOPUS:85084155628
SN - 1350-4533
VL - 80
SP - 52
EP - 59
JO - Medical Engineering and Physics
JF - Medical Engineering and Physics
ER -