Single-shot Mueller-matrix imaging of zebrafish tissues: In vivo analysis of developmental and pathological features

Alexander Machikhin, Chih Chung Huang, Demid Khokhlov, Victoria Galanova, Alexander Burlakov

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Zebrafish is a well-established animal model for developmental and disease studies. Its optical transparency at early developmental stages allows in vivo tissues visualization. Interaction of polarized light with these tissues provides information on their structure and properties. This approach is effective for muscle tissue analysis due to its birefringence. To enable real-time Mueller-matrix characterization of unanesthetized fish, we assembled a microscope for single-shot Mueller-matrix imaging. First, we performed a continuous observation of 48 species within the period of 2 to 96 hpf and measured temporal dependencies of the polarization features in different tissues. These measurements show that hatching was accompanied by a sharp change in the angle and degree of linearly polarized light after interaction with muscles. Second, we analyzed nine species with skeletal disorders and demonstrated that the spatial distribution of light depolarization features clearly indicated them. Obtained results demonstrated that real-time Mueller-matrix imaging is a powerful tool for label-free monitoring zebrafish embryos.

原文English
文章編號e202200088
期刊Journal of Biophotonics
15
發行號9
DOIs
出版狀態Published - 2022 9月

All Science Journal Classification (ASJC) codes

  • 一般化學
  • 一般材料科學
  • 一般生物化學,遺傳學和分子生物學
  • 一般工程
  • 一般物理與天文學

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