TY - JOUR
T1 - Site-directed mutagenesis of Asp280 suggests substrate-assisted catalysis of chitinase Al from Bacillus circulans WL-12
AU - Hashimoto, Masayuki
AU - Honda, Yuji
AU - Nikaidou, Naoki
AU - Fukamizo, Tamo
AU - Watanabe, Takeshi
PY - 2000
Y1 - 2000
N2 - To study the involvement of Asp280 of chitinase A1 from Bacillus circulans WL-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. Kinetic analysis of the mutant chitinases revealed that the residue is not absolutely essential for the catalytic reaction and thus, does not act as the catalytic nucleophile.
AB - To study the involvement of Asp280 of chitinase A1 from Bacillus circulans WL-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. Kinetic analysis of the mutant chitinases revealed that the residue is not absolutely essential for the catalytic reaction and thus, does not act as the catalytic nucleophile.
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U2 - 10.1016/s1389-1723(00)90031-8
DO - 10.1016/s1389-1723(00)90031-8
M3 - Article
C2 - 16232709
AN - SCOPUS:0033974048
SN - 1389-1723
VL - 89
SP - 100
EP - 102
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -