SLCO3A1, a novel Crohn's disease-associated gene, regulates NF-κB activity and associates with intestinal perforation

Shu Chen Wei, Yan Yin Tan, Meng Tzu Weng, Liang Chuan Lai, Jen Hao Hsiao, Eric Y. Chuang, Chia Tung Shun, Deng Cheng Wu, Ai Wen Kao, Chiao Shung Chuang, Yen Hsuan Ni, Ming Jium Shieh, Chien Chih Tung, Yun Chen, Cheng Yi Wang, Ramnik J. Xavier, Daniel K. Podolsky, Jau Min Wong

研究成果: Article同行評審

15 引文 斯高帕斯(Scopus)


Background & Aims: To date, only one gene (TNFSF15) has been identified and validated as a Crohn's disease (CD)-associated gene in non-Caucasian populations. This study was designed to identify novel CD-associated single nucleotide polymorphisms (SNPs)/genes and to validate candidate genes using a functional assay. Methods: SNPs from 16 CD patients and 16 age- and sex-matched control patients were analyzed using Illumina platform analysis. Subsequently, we expanded the study and followed 53 CD patients and 41 control patients by Sequenom MassArray analysis. Quantitative PCR and immunohistochemical staining were performed to assess mRNA and protein expression of the candidate gene on tissue isolated from CD patients. Genotype was correlated with CD phenotypes. Finally, the candidate gene was cloned and its effect on NF-κB activity assessed using a reporter luciferase assay. Results: SLCO3A1 (rs207959) reached statistical significance in the first-stage analysis ( P = 2.3E-02) and was further validated in the second-stage analysis (P = 1.0E-03). Genotype and phenotype analysis showed that the rs207959 (T) allele is a risk allele that alters SLCO3A1 mRNA expression and is associated with intestinal perforation in CD patients. Higher levels of mRNA and protein expression of SLCO3A1 were seen in CD patients compared with the control group. Overexpression of SLCO3A1 induced increased NF-κB activity and increased phosphorylation of P65, ERK, and JNK. Nicotine augmented the activation of NF-κB in the presence of SLCO3A1. Conclusions: SLCO3A1, a novel CD-associated gene, mediates inflammatory processes in intestinal epithelial cells through NF-κB transcription activation, resulting in a higher incidence of bowel perforation in CD patients.

期刊PloS one
出版狀態Published - 2014 6月 19

All Science Journal Classification (ASJC) codes

  • 生物化學、遺傳與分子生物學 (全部)
  • 農業與生物科學 (全部)
  • 多學科


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