Slug is temporally regulated by cyclin e in cell cycle and controls genome stability

W. L. Wang, H. C. Huang, S. H. Kao, Y. C. Hsu, Y. T. Wang, K. C. Li, Y. J. Chen, S. L. Yu, S. P. Wang, T. H. Hsiao, P. C. Yang, Tse-Ming Hong

研究成果: Article

13 引文 (Scopus)

摘要

The transcriptional repressor Slug is best known to control epithelial-mesenchymal transition (EMT) and promote cancer invasion/metastasis. In this study, we demonstrate that Slug is temporally regulated during cell cycle progression. At G1/S transition, cyclin E-cyclin-dependent kinase 2 mediates the phosphorylation of Slug at Ser-54 and Ser-104, resulting in its ubiquitylation and degradation. Non-phosphorylatable Slug is markedly stabilized at G1/S transition compared with wild-type Slug and greatly leads to downregulation of DNA synthesis and checkpoint-related proteins, including TOP1, DNA Ligase IV and Rad17, reduces cell proliferation, delays S-phase progression and contributes to genome instability. Our results indicate that Slug has multifaceted roles in cancer progression by controlling both EMT and genome stability.

原文English
頁(從 - 到)1116-1125
頁數10
期刊Oncogene
34
發行號9
DOIs
出版狀態Published - 2015 二月 26

指紋

Gastropoda
Cyclins
Genomic Instability
Cell Cycle Checkpoints
Epithelial-Mesenchymal Transition
Cyclin-Dependent Kinase 2
Cyclin E
Ubiquitination
S Phase
Neoplasms
Cell Cycle
Down-Regulation
Phosphorylation
Cell Proliferation
Neoplasm Metastasis
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

引用此文

Wang, W. L., Huang, H. C., Kao, S. H., Hsu, Y. C., Wang, Y. T., Li, K. C., ... Hong, T-M. (2015). Slug is temporally regulated by cyclin e in cell cycle and controls genome stability. Oncogene, 34(9), 1116-1125. https://doi.org/10.1038/onc.2014.58
Wang, W. L. ; Huang, H. C. ; Kao, S. H. ; Hsu, Y. C. ; Wang, Y. T. ; Li, K. C. ; Chen, Y. J. ; Yu, S. L. ; Wang, S. P. ; Hsiao, T. H. ; Yang, P. C. ; Hong, Tse-Ming. / Slug is temporally regulated by cyclin e in cell cycle and controls genome stability. 於: Oncogene. 2015 ; 卷 34, 編號 9. 頁 1116-1125.
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abstract = "The transcriptional repressor Slug is best known to control epithelial-mesenchymal transition (EMT) and promote cancer invasion/metastasis. In this study, we demonstrate that Slug is temporally regulated during cell cycle progression. At G1/S transition, cyclin E-cyclin-dependent kinase 2 mediates the phosphorylation of Slug at Ser-54 and Ser-104, resulting in its ubiquitylation and degradation. Non-phosphorylatable Slug is markedly stabilized at G1/S transition compared with wild-type Slug and greatly leads to downregulation of DNA synthesis and checkpoint-related proteins, including TOP1, DNA Ligase IV and Rad17, reduces cell proliferation, delays S-phase progression and contributes to genome instability. Our results indicate that Slug has multifaceted roles in cancer progression by controlling both EMT and genome stability.",
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Wang, WL, Huang, HC, Kao, SH, Hsu, YC, Wang, YT, Li, KC, Chen, YJ, Yu, SL, Wang, SP, Hsiao, TH, Yang, PC & Hong, T-M 2015, 'Slug is temporally regulated by cyclin e in cell cycle and controls genome stability', Oncogene, 卷 34, 編號 9, 頁 1116-1125. https://doi.org/10.1038/onc.2014.58

Slug is temporally regulated by cyclin e in cell cycle and controls genome stability. / Wang, W. L.; Huang, H. C.; Kao, S. H.; Hsu, Y. C.; Wang, Y. T.; Li, K. C.; Chen, Y. J.; Yu, S. L.; Wang, S. P.; Hsiao, T. H.; Yang, P. C.; Hong, Tse-Ming.

於: Oncogene, 卷 34, 編號 9, 26.02.2015, p. 1116-1125.

研究成果: Article

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AU - Wang, Y. T.

AU - Li, K. C.

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Wang WL, Huang HC, Kao SH, Hsu YC, Wang YT, Li KC 等. Slug is temporally regulated by cyclin e in cell cycle and controls genome stability. Oncogene. 2015 2月 26;34(9):1116-1125. https://doi.org/10.1038/onc.2014.58