SNARE Proteins Synaptobrevin, SNAP-25, and Syntaxin Are Involved in Rapid and Slow Endocytosis at Synapses

Jianhua Xu, Fujun Luo, Zhen Zhang, Lei Xue, Xin Sheng Wu, Hsueh Cheng Chiang, Wonchul Shin, Ling Gang Wu

研究成果: Article同行評審

62 引文 斯高帕斯(Scopus)

摘要

Rapid endocytosis, which takes only a few seconds, is widely observed in secretory cells. Although it is more efficient in recycling vesicles than in slow clathrin-mediated endocytosis, its underlying mechanism, thought to be clathrin independent, is largely unclear. Here, we report that cleavage of three SNARE proteins essential for exocytosis, including synaptobrevin, SNAP-25, and syntaxin, inhibited rapid endocytosis at the calyx of Held nerve terminal, suggesting the involvement of the three SNARE proteins in rapid endocytosis. These SNARE proteins were also involved in slow endocytosis. In addition, SNAP-25 and syntaxin facilitated vesicle mobilization to the readily releasable pool, most likely via theirroles in endocytosis and/or exocytosis. We conclude that both rapid and slow endocytosis share the involvement of SNARE proteins. The dual role ofthree SNARE proteins in exo- and endocytosis suggests that SNARE proteins may be molecular substrates contributing to the exocytosis-endocytosis coupling, which maintains exocytosis in secretory cells.

原文English
頁(從 - 到)1414-1421
頁數8
期刊Cell Reports
3
發行號5
DOIs
出版狀態Published - 2013 5月 30

All Science Journal Classification (ASJC) codes

  • 生物化學、遺傳與分子生物學 (全部)

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