Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)

Pei Phen Liu, Yen Chin Chen, Ching Li, Yu Huei Hsieh, Shu Wan Chen, Shu Huei Chen, Wen Yih Jeng, Woei Jer Chuang

研究成果: Article同行評審

18 引文 斯高帕斯(Scopus)

摘要

Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra α-helix. This is the first study to report the presence of a C-terminal α-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding.

原文English
頁(從 - 到)543-553
頁數11
期刊Proteins: Structure, Function and Genetics
49
發行號4
DOIs
出版狀態Published - 2002 十二月 1

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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