Solution structure of the fibronectin type III domain from Bacillus circulans WL-12 chitinase A1

Jun Goo Jee, Takahisa Ikegami, Masayuki Hashimoto, Takeshi Kawabata, Mitsunori Ikeguchi, Takeshi Watanabe, Masahiro Shirakawa

研究成果: Article同行評審

48 引文 斯高帕斯(Scopus)

摘要

Growing evidence suggests that horizontal gene transfer plays an integral role in the evolution of bacterial genomes. One of the debated examples of horizontal gene transfer from animal to prokaryote is the fibronectin type III domain (FnIIID). Certain extracellular proteins of soil bacteria contain an unusual cluster of Fn-IIIDs, which show sequence similarity to those of animals and are likely to have been acquired horizontally from animals. Here we report the solution structure of the FnIIID of chitinase A1 from Bacillus circulans WL-12. To the best of our knowledge, this is the first tertiary structure to be reported for an FnIIID from a bacterial protein. The structure of the domain shows significant similarity to FnIIIDs from animal proteins. Sequence comparisons with FnIIIDs from other soil bacteria proteins show that the core-forming residues are highly conserved and, thus, are under strong evolutionary pressure. Striking similarities in the tertiary structures of bacterial FnIIIDs and their mammalian counterparts may support the hypothesis that the evolution of the FnIIID in bacterial carbohydrases occurred horizontally. The total lack of surface-exposed aromatic residues also suggests that the role of this FnIIID is different from those of other bacterial β-sandwich domains, which function as carbohydrate-binding modules.

原文English
頁(從 - 到)1388-1397
頁數10
期刊Journal of Biological Chemistry
277
發行號2
DOIs
出版狀態Published - 2002 一月 11

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學
  • 細胞生物學

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