Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning of thick tissues

Li Chung Cheng, Chia Yuan Chang, Wei Chung Yen, Shean Jen Chen

研究成果: Conference contribution

3 引文 斯高帕斯(Scopus)


Conventional multiphoton microscopy employs beam scanning; however, in this study a microscope based on spatiotemporal focusing offering widefield multiphoton excitation has been developed to provide fast optical sectioning images. The microscope integrates a 10 kHz repetition rate ultrafast amplifier featuring strong instantaneous peak power (maximum 400 μJ/pulse at 90 fs pulse width) with a TE-cooled, ultra-sensitive photon detecting, electron multiplying charge-coupled device camera. This configuration can produce multiphoton excited images with an excitation area larger than 200 × 100 μm2 at a frame rate greater than 100 Hz. Brownian motions of fluorescent microbeads as small as 0.5 μm have been instantaneously observed with a lateral spatial resolution of less than 0.5 μm and an axial resolution of approximately 3.5 μm. Moreover, we combine the widefield multiphoton microscopy with structure illuminated technique named HiLo to reject the background scattering noise to get better quality for bioimaging.

主出版物標題Unconventional Imaging and Wavefront Sensing 2012
出版狀態Published - 2012 12月 1
事件Unconventional Imaging and Wavefront Sensing 2012 - San Diego, CA, United States
持續時間: 2012 8月 132012 8月 14


名字Proceedings of SPIE - The International Society for Optical Engineering


ConferenceUnconventional Imaging and Wavefront Sensing 2012
國家/地區United States
城市San Diego, CA

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 凝聚態物理學
  • 電腦科學應用
  • 應用數學
  • 電氣與電子工程


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