Streptococcal pyrogenic exotoxin B cleaves human S-adenosylhomocysteine hydrolase and induces hypermethioninemia

Ju Fei Hsu, Woei Jer Chuang, Shu Chu Shiesh, Yee Shin Lin, Ching Chuan Liu, Chih Chieh Wang, Tzu N. Fu, Jui He Tsai, Wei Lun Tsai, Yu Jun Huang, Yi Hsuan Hsieh, Jiunn Jong Wu, Ming T. Lin, Wenya Huang

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4 引文 斯高帕斯(Scopus)

摘要

Group A Streptococcus is a common pathogen that causes pharyngitis, impetigo, myositis, and lethal streptococcal toxic shock syndrome. Streptococcal pyrogenic exotoxin B (SPE B) is strongly associated with the severity of disease. SPE B is a cysteine protease and matures itself by autocatalysis. We found that SPE B was directly associated with human S-adenosylhomocysteine hydrolase (AdoHcyase), an essential factor for a delayed-type immune response. AdoHcyase protein levels and enzymatic activities were significantly higher in human cells infected with the Streptococcus pyogenes SW510 speB mutant strain than in cells infected with the NZ131 wild-type strain. SPE B also inactivated AdoHcyase, shown by a decrease in homocysteine, the main product of AdoHcyase. We found that in vivo and in vitro, SPE B induced hypermethioninemia, which is caused by an AdoHcyase defect. We also found that AdoHcyase is a substrate of SPE B cysteine protease. SPE B, therefore, potentially causes immunosuppression by cleaving AdoHcyase.

原文English
頁(從 - 到)367-374
頁數8
期刊Journal of Infectious Diseases
198
發行號3
DOIs
出版狀態Published - 2008 八月 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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