Streptomycin and its analogues are potent inhibitors of the hypotonicity-induced Ca2+ entry and Cl- channel activity

研究成果: Article同行評審

17 引文 斯高帕斯(Scopus)

摘要

Streptomycin is a common antibiotic used in culture media. It is also a known blocker of stretch-activated and mechanosensitive ion channels in neurons and cardiac myocytes. But very little information is available on its effect in the regulation of epithelial ion channels. Osmotic swelling is a kind of mechanical stretch. The opening of stretch-activated Ca2+ channels contributes to hypotonicity-induced Ca2+ influx which is necessary for the activation of volume-regulated Cl- channels in human cervical cancer cells. This study aimed to investigate the role of streptomycin in cell volume regulation. Treatment of cervical cancer SiHa cells with streptomycin and its analogues (gentamicin and netilmicin) did not affect the basal cytosolic Ca2+ ([Ca2+]i) level. But it attenuated the hypotonicity-stimulated increase of [Ca2+] i in a dose-dependent manner with half-maximal inhibitory concentrations (IC50) of 25, 90 and 200 μM for streptomycin, gentamicin and netilmicin, respectively, when measured at room temperature. In contrast, under free extracellular Ca2+ condition, hypotonic stress only induced a small, progressive increase of [Ca2+]i, while 500 μM streptomycin did not affect this Ca2+ signaling. Streptomycin and its analogues (gentamicin and netilmicin) also inhibited the activation of volume-regulated Cl- channels in a dose-dependent manner with IC50 of 30, 95 and 250 μM at room temperature, respectively. Chronic culture with 50 μM streptomycin downregulates the activity of volume-regulated Cl- channels and retards the process of regulatory volume decrease in SiHa cells and MDCK cells. We suggest that using cells chronically cultured with streptomycin to study epithelial ion channels risks studying cellular and molecular pathology rather than physiology.

原文English
頁(從 - 到)494-500
頁數7
期刊FEBS Letters
554
發行號3
DOIs
出版狀態Published - 2003 十一月 20

All Science Journal Classification (ASJC) codes

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 分子生物學
  • 遺傳學
  • 細胞生物學

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