Structural insights into the interaction between phytoplasmal effector causing phyllody 1 and MADS transcription factors

Yi Ting Liao, Shih Shun Lin, Shin Jen Lin, Wan Ting Sun, Bing Nan Shen, Han Pin Cheng, Chan Pin Lin, Tzu Ping Ko, Yi Fan Chen, Hao Ching Wang

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)


Phytoplasmas are bacterial plant pathogens which can induce severe symptoms including dwarfism, phyllody and virescence in an infected plant. Because phytoplasmas infect many important crops such as peanut and papaya they have caused serious agricultural losses. The phytoplasmal effector causing phyllody 1 (PHYL1) is an important phytoplasmal pathogenic factor which affects the biological function of MADS transcription factors by interacting with their K (keratin-like) domain, thus resulting in abnormal plant developments such as phyllody. Until now, lack of information on the structure of PHYL1 has prevented a detailed understanding of the binding mechanism between PHYL1 and the MADS transcription factors. Here, we present the crystal structure of PHYL1 from peanut witches’-broom phytoplasma (PHYL1Pn WB). This protein was found to fold into a unique α-helical hairpin with exposed hydrophobic residues on its surface that may play an important role in its biological function. Using proteomics approaches, we propose a binding mode of PHYL1Pn WB with the K domain of the MADS transcription factor SEPALLATA3 (SEP3_K) and identify the residues of PHYL1Pn WB that are important for this interaction. Furthermore, using surface plasmon resonance we measure the binding strength of PHYL1Pn WB proteins to SEP3_K. Lastly, based on confocal images, we found that α-helix 2 of PHYL1Pn WB plays an important role in PHYL1-mediated degradation of SEP3. Taken together, these results provide a structural understanding of the specific binding mechanism between PHYL1Pn WB and SEP3_K.

頁(從 - 到)706-719
期刊Plant Journal
出版狀態Published - 2019 11月 1

All Science Journal Classification (ASJC) codes

  • 遺傳學
  • 植物科學
  • 細胞生物學


深入研究「Structural insights into the interaction between phytoplasmal effector causing phyllody 1 and MADS transcription factors」主題。共同形成了獨特的指紋。