Structural Properties of CXCL4L1 and Its Recognition of the CXCR3 N-Terminus

Ya Hsin Liu, Chia Hsuan Chuang, Yi Zong Lee, Eh Tzen Lee, Chiao Ling Lo, Chu Ya Wu, Li Kun Huang, Andreas Bikfalvi, Shih Che Sue

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

Chemokine CXCL4L1, a homologue of CXCL4, is a more potent antiangiogenic ligand. Its structural property is correlated with the downstream receptor binding. The two chemokines execute their functions by binding the receptors of CXCR3A and CXCR3B. The receptors differ by an extra 51-residue extension in the CXCR3B N-terminus. To understand the binding specificity, a GB1 protein scaffold was used to carry different CXCR3 extracellular elements, and artificial CXCL4 and CXCL4L1 monomers were engineered for the binding assay. We first characterized the molten globule property of CXCL4L1. The structural property causes the CXCL4L1 tetramer to dissociate into monomers in low concentrations, but native CXCL4 adopts a stable tetramer structure in solution. In the titration experiments, the combination of the CXCR3A N-terminus and receptor extracellular loop 2 provided moderate and comparable binding affinities to CXCL4 and CXCL4L1, while sulfation on the CXCR3A N-terminal tyrosine residues provided binding specificity. However, the CXCR3B N-terminal extension did not show significant enhancement in the binding of CXCL4 or CXCL4L1. This result indicates that the tendency to form a chemokine monomer and the binding affinity together contribute the high antiangiogenic activity of CXCL4L1.

原文English
頁(從 - 到)722-734
頁數13
期刊Biochemistry
62
發行號3
DOIs
出版狀態Published - 2023 2月 7

All Science Journal Classification (ASJC) codes

  • 生物化學

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