Study of cell transmigration using a co-culture microsystem under shear stress

C. H. Yeh, S. H. Tsai, Y. H. Huang, L. W. Wu, Y. C. Lin

研究成果: Conference article同行評審

1 引文 斯高帕斯(Scopus)

摘要

This study reports a novel cell co-culture technique using micro-molding in capillaries (MIMIC) technology that was utilized to observe the transmigration conditions of two types of cells with and without fluidic shear stress. Besides, the gap size of co-culture device could achieve shortest and not mixture. Endothelial cells (ECs) and smooth muscle cells (SMCs) were used in our experiment. In addition, concentrations of two cell are 8000 cells/μL (ECs) and 9000 cells/μL (SMCs), respectively, the shear stress is 7 dyne/cm2, and the isolation distance between two types of cell are 50 and 200 μm. It is found that in the smaller culture space (50 μm) condition, ECs and SMCs would induce mutually, which would further make cell migration; in larger culture space (200 μm) condition, no inducing reaction took place between ECs and SMCs. It will have more advantages in bio-manipulation and tissue repair engineering.

原文English
頁(從 - 到)361-364
頁數4
期刊Procedia Chemistry
1
發行號1
DOIs
出版狀態Published - 2009 九月
事件Eurosensors 23rd Conference - Lausanne, Switzerland
持續時間: 2009 九月 62009 九月 9

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 化學工程 (全部)

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