Study of cholesterol's effect on the properties of catanionic vesicular systems: Comparison of light-scattering results with ultrasonic and fluorescence spectroscopy

Martina Havlíková, Jana Szabová, Adam Jugl, Ludmila Mravcová, Chien Hsiang Chang, Po Sung Huang, Miloslav Pekař, Filip Mravec

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

This work is focused on the study of properties associated with the effect of cholesterol levels on the stability of vesicular systems based on the ion pair amphiphile hexadecyltrimethylammonium-dodecylsulphate (HTMA-DS) at laboratory temperature. The HTMA-DS catanionic system was doped with dioctadecyldimethylammonium chloride in a 9:1 M ratio and cholesterol in the amount of 0, 3, 13, 23, 33, 43, 53, 63, and 73 mol.% was added. In this system, the size distributions were studied using the dynamic light-scattering technique and the zeta potential was determined. These standard techniques were supplemented by ultrasonic and fluorescence spectroscopy techniques. Due to low stability and high opalescence of samples, spectral techniques were used only for the samples with cholesterol content above 23 mol.%. The results from High-Resolution Ultrasonic Spectroscopy and from Fluorescence Spectroscopy are in agreement. They equally point to a change in the amount of hydration water in the membrane, the largest amount of which is present in the samples with 43 and 53 mol.% cholesterol. Using the light-scattering technique, the short-term stability of prepared vesicular systems was also observed over the first 36 days. Obtained results confirmed that the most stable systems are those containing 43 or 53 mol.% of cholesterol.

原文English
文章編號125526
期刊Colloids and Surfaces A: Physicochemical and Engineering Aspects
607
DOIs
出版狀態Published - 2020 十二月 20

All Science Journal Classification (ASJC) codes

  • 表面和介面
  • 物理與理論化學
  • 膠體和表面化學

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