Kaiser effect is a widely investigated laboratory testing method combining Acoustic Emission technology with uniaxial compressive test to determine the pre-stress of a rock. This paper provides a quantitatively discussions on the influences of anisotropy on the accuracy of pre-stress determination with different stress levels. The results show that rock anisotropy has insignificant influences on the accuracy of pre-stress determination. In addition, a rock with high stress state has low error for the pre-stress assessment. However, more studies are required to investigate the applicability of AE method to estimate the low pre-stress for the samples having different dip angles of schistosity.