SU-8 based continuous-flow RT-PCR bio-chips under high-precision temperature control

Nan Chyuan Tsai, Chung Yang Sue

研究成果: Article同行評審

33 引文 斯高帕斯(Scopus)

摘要

Being beneficial from dramatic progress in bio-chemical and micro-electro-mechanical technologies, DNA manipulation devices can be miniaturized to expedite amplification processing for virus detection. Polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) are two typical examples of them. In this report, a micro-RT-PCR (μRT-PCR) chip is designed to quantitatively detect tumor viruses. Test sample reservoirs, RT-PCR meanders, and capillary electrophoresis are integrated on a SU-8 based monolithic chip. A high-precision temperature control module is well developed by embedding signal amplification circuits and an Intel 8051 microprocessor. The integrated system exhibits high efficacy for heat transfer, exemption of fluid flow clogging and superior sensitivity to precisely control the required reaction temperatures in all heated zones of μRT-PCR unit. Intensive simulations and experiments are presented to verify the validity of the fabricated bio-chip.

原文English
頁(從 - 到)313-317
頁數5
期刊Biosensors and Bioelectronics
22
發行號2
DOIs
出版狀態Published - 2006 八月 15

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物物理學
  • 生物醫學工程
  • 電化學

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