Substrate specificities of Escherichia coli thioesterase I/protease I/lysophospholipase L1 are governed by its switch loop movement

Yu Chih Lo, Su Chang Lin, Jei Fu Shaw, Yen Chywan Liaw

研究成果: Article同行評審

22 引文 斯高帕斯(Scopus)

摘要

Escherichia coli thioesterase I/protease I/lysophospholipase L1 (TAP) is a multifunctional lysophospholipase and acyl-CoA thioesterase with a SGNH-hydrolase fold. The relationship between TAP's structure and its versatile substrate specificity, however, is unclear. Here, we present the crystal structure of TAP in complex with octanoic acid (TAP-OCA; OCA, a free fatty acid with eight carbon atoms, C8). A structural comparison of native TAP with TAP-OCA reveals a remarkable conformational change in loop75-80, called "switch loop movement", upon OCA binding to the substrate-binding crevice of TAP. OCA binding to the substrate-binding crevice results in a continuous hydrophobic surface, which triggers switch loop movement. The switch loop movement is acyl chain length dependent, with an effect of stabilizing the Michaelis complex (MC) of TAP during catalysis, and is essential for TAP's substrate preference. The finding of a sulfate ion binding site in the TAP structures, together with previous enzyme kinetic analyses, leads us to postulate that a putative CoA binding site is essential for efficient catalysis of thioesters in TAP. We also present the crystal structure of L109P-OCA (TAP's L109P mutant in complex with OCA), in which Leu109 mutated to Pro109 abolishes switch loop movement. This result strengthens our hypothesis that the switch loop movement is induced by hydrophobic interactions.

原文English
頁(從 - 到)1971-1979
頁數9
期刊Biochemistry
44
發行號6
DOIs
出版狀態Published - 2005 二月 15

All Science Journal Classification (ASJC) codes

  • Biochemistry

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