Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair

William J. Feaver, Wenya Huang, Opher Gileadi, Lawrence Myers, Claes M. Gustafsson, Roger D. Kornberg, Errol C. Friedberg

研究成果: Article同行評審

21 引文 斯高帕斯(Scopus)

摘要

A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.

原文English
頁(從 - 到)5941-5946
頁數6
期刊Journal of Biological Chemistry
275
發行號8
DOIs
出版狀態Published - 2000 2月 25

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學
  • 細胞生物學

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