Suction-type microfluidic immunosensing systems for rapid detection of dengue fever

C. H. Weng, T. B. Huang, C. C. Huang, C. S. Yeh, H. Y. Lei, G. B. Lee

研究成果: Conference contribution

摘要

This study reports an integrated microfluidic system for rapid immunosensing of antibodies associated with dengue fever by utilizing enzyme-linked immunoabsorbent assay (ELISA) poly(dimethylsiloxane) (PDMS)-based chip. In this study, we successfully developed a rapid immunosensing chip with pre-functionalized surfaces to avoid protein adsorption. The functionalized PDMS surface was found to be highly effective in reducing the adsorption time in microchannels. The functionalized surfaces can be stable at least for 1 month when stored at 4°C. In addition, a suction-type microfluidic transportation module was used to form a single-fluid-slug transportation and to mix the reagents for rapid detection of dengue virus. The entire process can be finished within 30 min. The sensitivity was found to be superior to the conventional methods.

原文English
主出版物標題14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
頁面97-99
頁數3
出版狀態Published - 2010
事件14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
持續時間: 2010 10月 32010 10月 7

出版系列

名字14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
1

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
國家/地區Netherlands
城市Groningen
期間10-10-0310-10-07

All Science Journal Classification (ASJC) codes

  • 控制與系統工程

指紋

深入研究「Suction-type microfluidic immunosensing systems for rapid detection of dengue fever」主題。共同形成了獨特的指紋。

引用此