Surface modification of iron oxide nanoparticles with polyarginine as a highly positively charged magnetic nano-adsorbent for fast and effective recovery of acid proteins

Bo Hung Lai, Chun Chieh Yeh, Dong Hwang Chen

研究成果: Article同行評審

57 引文 斯高帕斯(Scopus)

摘要

Polyarginine has been successfully bound onto iron oxide nanoparticles via carbodiimide activation as a highly positively charged magnetic nano-adsorbent for protein separation. They were nearly superparamagnetic with a mean diameter of 10.3 ± 2.36 nm, and the binding process did not change the spinel structure of iron oxide. From the analyses of FTIR spectra and zeta potential, the binding of polyarginine on the surface of iron oxide was confirmed and the resultant polyarginine-coated magnetic nanoparticles (PA-MNPs) were positively charged even up to pH 11. By thermogravimetric analysis, the typical product contained about 7.1 wt% of polyarginine. From the adsorption of the proteins with different pI values, the resultant PA-MNPs were found to be quite efficient for the fast and effective adsorption of acid proteins. For the typical acid protein, bovine serum albumin (BSA), the adsorption equilibrium was achieved within few minutes and obeyed the Langmuir isotherm equation. At pH 7 and 25 °C, the maximum adsorption capacity and equilibrium constant were 67.6 mg/g and 0.0623 L/mg, respectively. Moreover, by SDS-polyacrylamide gel electrophoresis, the capability of PA-MNPs for the separation of BSA-lysozyme mixture and egg white was further confirmed. Accordingly, the PA-MNPs were useful for the fast and effective magnetic recovery of acid proteins.

原文English
頁(從 - 到)799-805
頁數7
期刊Process Biochemistry
47
發行號5
DOIs
出版狀態Published - 2012 5月

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物化學
  • 應用微生物與生物技術

指紋

深入研究「Surface modification of iron oxide nanoparticles with polyarginine as a highly positively charged magnetic nano-adsorbent for fast and effective recovery of acid proteins」主題。共同形成了獨特的指紋。

引用此