Surface plasmon-enhanced and quenched two-photon excited fluorescence

C. Y. Lin, C. H. Lien, K. C. Chiu, C. Y. Chang, S. H. Chang, T. F. Guo, S. J. Chen

研究成果: Conference contribution

摘要

This study investigated theoretically and experimentally that two-photon excited fluorescence is enhanced and quenched via surface plasmons (SPs) excited by total internal reflection with a silver film. The fluorescence intensity is fundamentally affected by the local electromagnetic field enhancement and the quantum yield change according to the surrounding structure and materials. By utilizing the Fresnel equation and classical dipole radiation modeling, local electric field enhancement, fluorescence quantum yield, and fluorescence emission coupling yield via SPs were theoretically analyzed at different dielectric spacer thicknesses between the fluorescence dye and the metal film. The fluorescence lifetime was also decreased substantially via the quenching effect. A two-photon excited total internal reflection fluorescence (TIRF) microscopy with a time-correlated single photon counting device has been developed to measure the fluorescence lifetimes, photostabilities, and enhancements. The experimental results demonstrate that the fluorescence lifetimes and the trend of the enhancements are consistent with the theoretical analysis. The maximum fluorescence enhancement factor in the surface plasmon-total internal reflection fluorescence (SP-TIRF) configuration can be increased up to 30 fold with a suitable thickness SiO2 spacer. Also, to compromise for the fluorescence enhancement and the fluorophore photostability, we find that the SP-TIRF configuration with a 10 nm SiO 2 spacer can provide an enhanced and less photobleached fluorescent signal via the assistance of enhanced local electromagnetic field and quenched fluorescence lifetime, respectively.

原文English
主出版物標題Plasmonics
主出版物子標題Metallic Nanostructures and Their Optical Properties VIII
DOIs
出版狀態Published - 2010 十二月 1
事件Plasmonics: Metallic Nanostructures and Their Optical Properties VIII - San Diego, CA, United States
持續時間: 2010 八月 12010 八月 5

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
7757
ISSN(列印)0277-786X

Other

OtherPlasmonics: Metallic Nanostructures and Their Optical Properties VIII
國家United States
城市San Diego, CA
期間10-08-0110-08-05

    指紋

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

引用此

Lin, C. Y., Lien, C. H., Chiu, K. C., Chang, C. Y., Chang, S. H., Guo, T. F., & Chen, S. J. (2010). Surface plasmon-enhanced and quenched two-photon excited fluorescence. 於 Plasmonics: Metallic Nanostructures and Their Optical Properties VIII [77573A] (Proceedings of SPIE - The International Society for Optical Engineering; 卷 7757). https://doi.org/10.1117/12.864042