摘要
A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.
| 原文 | English |
|---|---|
| 文章編號 | 71831L |
| 期刊 | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| 卷 | 7183 |
| DOIs | |
| 出版狀態 | Published - 2009 |
| 事件 | Multiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States 持續時間: 2009 1月 25 → 2009 1月 27 |
All Science Journal Classification (ASJC) codes
- 電子、光磁材料
- 生物材料
- 原子與分子物理與光學
- 放射學、核子醫學和影像學