Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

Ruei Yu He, Yuan Deng Su, Keng Chi Cho, Chun Yun Lin, Nan Shan Chang, Chih Han Chang, Shean Jen Chen

研究成果: Article同行評審

43 引文 斯高帕斯(Scopus)

摘要

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

原文English
頁(從 - 到)5987-5997
頁數11
期刊Optics Express
17
發行號8
DOIs
出版狀態Published - 2009 四月 13

All Science Journal Classification (ASJC) codes

  • 原子與分子物理與光學

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