Systems biology approach to exploring the effect of cyclic stretching on cardiac cell physiology

Chien Cheng Chen, Tzyy Yue Wong, Tzu Yun Chin, Wen Hsien Lee, Chan Yen Kuo, Yi Chiung Hsu

研究成果: Article同行評審

摘要

Although mechanical forces are involved in pressure-overloaded cardiomyopathy, their effects on gene transcription profiles are not fully understood. Here, we used next-generation sequencing (NGS) to investigate changes in genomic profiles after cyclic mechanical stretching of human cardiomyocytes. We found that 85, 87, 32, 29, and 28 genes were differentially expressed after 1, 4, 12, 24, and 48 hours of stretching. Furthermore, 10 of the 29 genes that were up-regulated and 11 of the 28 that were down-regulated after 24 h showed the same changes after 48 h. We then examined expression of the genes that encode serpin family E member 1 (SERPINE1), DNA-binding protein inhibitor 1 (ID1), DNA-binding protein inhibitor 3 (ID3), and CCL2, a cytokine that acts as chemotactic factor in monocytes, in an RT-PCR experiment. The same changes were observed for all four genes after all cyclic stretching durations, confirming the NGS results. Taken together, these findings suggest that cyclical stretching can alter cardiac cell physiology by activating cardiac cell metabolism and impacting cholesterol biosynthesis signaling.

原文English
頁(從 - 到)16035-16045
頁數11
期刊Aging
12
發行號16
DOIs
出版狀態Published - 2020 八月 31

All Science Journal Classification (ASJC) codes

  • 老化
  • 細胞生物學

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