Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor

Han Sheng Chuang, Jen Yi Wang, Jae Sung Kwon, Sheng Min Hsu

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

Bead-based immunosensing is a promising technology in the point-of-care diagnosis due to its high flexibility. To realize rapid and selective diagnosis, an optoelectrokinetic platyeform, termed rapid electrokinetic patterning (REP), was employed herein to drive a bead-based fluorescent immunosensor for diagnosis of diabetic retinopathy by lipocalin 1 (LCN1) and vascular epithelial growth factor (VEGF). The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The limit of detection (LOD) reached nearly 110 pg/mL. A DR phase plot was made to provide a clear way to determine whether a tested subject was under the threat of DR. At last, a preclinical evaluation was conducted. The accuracy of the proposed technique reached 94.74% in PDR and 89.47% in DM. The optoelectrokinetically driven bead-based immunosensor enables a noninvasive measure to the early diagnosis of diabetic retinopathy.

原文English
主出版物標題2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
發行者Institute of Electrical and Electronics Engineers Inc.
頁面1124-1127
頁數4
ISBN(電子)9781728120072
DOIs
出版狀態Published - 2019 六月
事件20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
持續時間: 2019 六月 232019 六月 27

出版系列

名字2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
國家/地區Germany
城市Berlin
期間19-06-2319-06-27

All Science Journal Classification (ASJC) codes

  • 製程化學與技術
  • 光譜
  • 電氣與電子工程
  • 機械工業
  • 電子、光磁材料
  • 控制和優化
  • 儀器

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