Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor

Han Sheng Chuang, Jen Yi Wang, Jae Sung Kwon, Sheng Min Hsu

研究成果: Conference contribution

摘要

Bead-based immunosensing is a promising technology in the point-of-care diagnosis due to its high flexibility. To realize rapid and selective diagnosis, an optoelectrokinetic platyeform, termed rapid electrokinetic patterning (REP), was employed herein to drive a bead-based fluorescent immunosensor for diagnosis of diabetic retinopathy by lipocalin 1 (LCN1) and vascular epithelial growth factor (VEGF). The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The limit of detection (LOD) reached nearly 110 pg/mL. A DR phase plot was made to provide a clear way to determine whether a tested subject was under the threat of DR. At last, a preclinical evaluation was conducted. The accuracy of the proposed technique reached 94.74% in PDR and 89.47% in DM. The optoelectrokinetically driven bead-based immunosensor enables a noninvasive measure to the early diagnosis of diabetic retinopathy.

原文English
主出版物標題2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
發行者Institute of Electrical and Electronics Engineers Inc.
頁面1124-1127
頁數4
ISBN(電子)9781728120072
DOIs
出版狀態Published - 2019 六月
事件20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
持續時間: 2019 六月 232019 六月 27

出版系列

名字2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
國家Germany
城市Berlin
期間19-06-2319-06-27

指紋

Immunosensors
Droplet
beads
Growth Factors
Patterning
Laser Beam
Electrode
Lipocalin 1
Electric Field
Flexibility
electrokinetics
Evaluation
Laser beams
Intercellular Signaling Peptides and Proteins
flexibility
plots
Electric fields
laser beams
Electrodes
electrodes

All Science Journal Classification (ASJC) codes

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

引用此文

Chuang, H. S., Wang, J. Y., Kwon, J. S., & Hsu, S. M. (2019). Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor. 於 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (頁 1124-1127). [8808207] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808207
Chuang, Han Sheng ; Wang, Jen Yi ; Kwon, Jae Sung ; Hsu, Sheng Min. / Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. 頁 1124-1127 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).
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abstract = "Bead-based immunosensing is a promising technology in the point-of-care diagnosis due to its high flexibility. To realize rapid and selective diagnosis, an optoelectrokinetic platyeform, termed rapid electrokinetic patterning (REP), was employed herein to drive a bead-based fluorescent immunosensor for diagnosis of diabetic retinopathy by lipocalin 1 (LCN1) and vascular epithelial growth factor (VEGF). The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The limit of detection (LOD) reached nearly 110 pg/mL. A DR phase plot was made to provide a clear way to determine whether a tested subject was under the threat of DR. At last, a preclinical evaluation was conducted. The accuracy of the proposed technique reached 94.74{\%} in PDR and 89.47{\%} in DM. The optoelectrokinetically driven bead-based immunosensor enables a noninvasive measure to the early diagnosis of diabetic retinopathy.",
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Chuang, HS, Wang, JY, Kwon, JS & Hsu, SM 2019, Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor. 於 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808207, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., 頁 1124-1127, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 19-06-23. https://doi.org/10.1109/TRANSDUCERS.2019.8808207

Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor. / Chuang, Han Sheng; Wang, Jen Yi; Kwon, Jae Sung; Hsu, Sheng Min.

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 1124-1127 8808207 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

研究成果: Conference contribution

TY - GEN

T1 - Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor

AU - Chuang, Han Sheng

AU - Wang, Jen Yi

AU - Kwon, Jae Sung

AU - Hsu, Sheng Min

PY - 2019/6

Y1 - 2019/6

N2 - Bead-based immunosensing is a promising technology in the point-of-care diagnosis due to its high flexibility. To realize rapid and selective diagnosis, an optoelectrokinetic platyeform, termed rapid electrokinetic patterning (REP), was employed herein to drive a bead-based fluorescent immunosensor for diagnosis of diabetic retinopathy by lipocalin 1 (LCN1) and vascular epithelial growth factor (VEGF). The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The limit of detection (LOD) reached nearly 110 pg/mL. A DR phase plot was made to provide a clear way to determine whether a tested subject was under the threat of DR. At last, a preclinical evaluation was conducted. The accuracy of the proposed technique reached 94.74% in PDR and 89.47% in DM. The optoelectrokinetically driven bead-based immunosensor enables a noninvasive measure to the early diagnosis of diabetic retinopathy.

AB - Bead-based immunosensing is a promising technology in the point-of-care diagnosis due to its high flexibility. To realize rapid and selective diagnosis, an optoelectrokinetic platyeform, termed rapid electrokinetic patterning (REP), was employed herein to drive a bead-based fluorescent immunosensor for diagnosis of diabetic retinopathy by lipocalin 1 (LCN1) and vascular epithelial growth factor (VEGF). The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The limit of detection (LOD) reached nearly 110 pg/mL. A DR phase plot was made to provide a clear way to determine whether a tested subject was under the threat of DR. At last, a preclinical evaluation was conducted. The accuracy of the proposed technique reached 94.74% in PDR and 89.47% in DM. The optoelectrokinetically driven bead-based immunosensor enables a noninvasive measure to the early diagnosis of diabetic retinopathy.

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U2 - 10.1109/TRANSDUCERS.2019.8808207

DO - 10.1109/TRANSDUCERS.2019.8808207

M3 - Conference contribution

AN - SCOPUS:85071926393

T3 - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

SP - 1124

EP - 1127

BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

PB - Institute of Electrical and Electronics Engineers Inc.

ER -

Chuang HS, Wang JY, Kwon JS, Hsu SM. Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor. 於 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 1124-1127. 8808207. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). https://doi.org/10.1109/TRANSDUCERS.2019.8808207