Temporal focusing-based multiphoton excitation fluorescence images with background noise cancellation via Hilbert-Huang transform

Yvonne Yuling Hu, Yuan Rong Luo, Chun Yu Lin, Chia-Yuan Chang, Shean Jen Chen

研究成果: Conference contribution

摘要

Temporal focusing multiphoton excitation microscopy has wide field-of-view and optical sectioning. By using digital micromirror device, it provides patterned illumination. However, without filling the back aperture of objective lens, the axial confinement is limited to micron-meters, leading the out-of-focus fluorophores excited and image blurred. In this study, Hilbert-Huang transform is proposed to reduce the background noise. The empirical mode decomposition is first applied to disassemble the image into intrinsic mode functions and then reconstruct by Hilbert transform after diminishing background residues. The axial confinement can be enhanced from 2.79 μm to 0.73 μm with structure frequency in 1.06 μm-1.

原文English
主出版物標題Three-Dimensional and Multidimensional Microscopy
主出版物子標題Image Acquisition and Processing XXVI
編輯Tony Wilson, Thomas G. Brown
發行者SPIE
ISBN(電子)9781510624085
DOIs
出版狀態Published - 2019 一月 1
事件Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI 2019 - San Francisco, United States
持續時間: 2019 二月 52019 二月 7

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
10883
ISSN(列印)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI 2019
國家/地區United States
城市San Francisco
期間19-02-0519-02-07

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 原子與分子物理與光學
  • 生物材料
  • 放射學、核子醫學和影像學

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